Rat, Human Predicted to work with:
Mouse, Horse, Cow, Dog, Rhesus monkey
Recombinant fragment corresponding to the N terminal amino acids 2-101 (AQPPRLSRSG ASSLWDPASP APTSGPRPRL WEGQDVLARW TDGLLYLGTI KKVDSAREVC LVQFEDDSQF LVLWKDISPA ALPGEELLCC VCRSETVVP) of Human PHF1 (NP_077084) with a proprietary tag.
Overlay histogram showing HeLa cells stained with ab87985 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab82247, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Western blot - PHF1 antibody [2G7] (ab87985)
Anti-PHF1 antibody [2G7] (ab87985) at 1 µg/ml + Immunogen at 0.1 µg
Secondary Goat anti mouse HRP at 5000 Developed using the ECL technique