Overview

  • Product name
  • Description
    Rabbit polyclonal to PHF1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog
  • Immunogen

    A region within synthetic peptide: PSPNQSYQGS SGYNFRPTDA RCLPSSPIRM FASFHPSAST AGTSGDSGPP, corresponding to C terminal amino acids 421-470 of Human PHF1

  • Positive control
    • 293T cell lysate, human stomach tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab66275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 34, (36), 48 kDa (predicted molecular weight: 62 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Function
    Transcriptional repressor. May promote methylation of histone H3 on 'Lys-27' by the PRC2/EED-EZH2 complex.
  • Tissue specificity
    Highest levels in heart, skeletal muscle, and pancreas, lower levels in brain, placenta, lung, liver and kidney.
  • Sequence similarities
    Contains 2 PHD-type zinc fingers.
  • Cellular localization
    Nucleus. Localizes specifically to the promoters of numerous target genes.
  • Information by UniProt
  • Database links
  • Alternative names
    • hPCl1 antibody
    • MTF2 L2 antibody
    • MTF2L 2 antibody
    • MTF2L2 antibody
    • PCL 1 antibody
    • PCL1 antibody
    • PHD finger protein 1 antibody
    • PHD finger protein1 antibody
    • PHF 1 antibody
    • PHF 2 antibody
    • Phf1 antibody
    • PHF1_HUMAN antibody
    • PHF2 antibody
    • Plc 1 antibody
    • Plc1 antibody
    • Polycomb like protein 1 antibody
    • Polycomb like protein1 antibody
    • Polycomb-like protein 1 antibody
    • Protein PHF1 antibody
    • T complex testis expressed 3 antibody
    • T complex testis expressed3 antibody
    • Tctex 3 antibody
    • Tctex3 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling PHF1 with ab66275 at 4-8µg/ml. Arrows indicate positively labelled surface mucous cells and epithelial cells of the fundic gland. Magnification: 400X.

  • Anti-PHF1 antibody (ab66275) at 1.25 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 62 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 34 kDa,36 kDa. We are unsure as to the identity of these extra bands.Left hand lane: molecular marker
    Gel concentration: 12%

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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