Recombinant
RabMAb

Anti-PHF1 antibody [EPR14222] (ab184951)

Overview

  • Product name
    Anti-PHF1 antibody [EPR14222]
    See all PHF1 primary antibodies
  • Description
    Rabbit monoclonal [EPR14222] to PHF1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PHF1 aa 300-400. The exact sequence is proprietary.
    Database link: O43189

  • Positive control
    • 293, Jurkat, MCF7 and Human fetal brain lysates; Human pancreas and Rat skeletal muscle tissues; MCF7 cells; 293 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
Flow Cyt 1/160.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/250 - 1/500.
WB 1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).

Target

  • Function
    Transcriptional repressor. May promote methylation of histone H3 on 'Lys-27' by the PRC2/EED-EZH2 complex.
  • Tissue specificity
    Highest levels in heart, skeletal muscle, and pancreas, lower levels in brain, placenta, lung, liver and kidney.
  • Sequence similarities
    Contains 2 PHD-type zinc fingers.
  • Cellular localization
    Nucleus. Localizes specifically to the promoters of numerous target genes.
  • Information by UniProt
  • Database links
  • Alternative names
    • hPCl1 antibody
    • MTF2 L2 antibody
    • MTF2L 2 antibody
    • MTF2L2 antibody
    • PCL 1 antibody
    • PCL1 antibody
    • PHD finger protein 1 antibody
    • PHD finger protein1 antibody
    • PHF 1 antibody
    • PHF 2 antibody
    • Phf1 antibody
    • PHF1_HUMAN antibody
    • PHF2 antibody
    • Plc 1 antibody
    • Plc1 antibody
    • Polycomb like protein 1 antibody
    • Polycomb like protein1 antibody
    • Polycomb-like protein 1 antibody
    • Protein PHF1 antibody
    • T complex testis expressed 3 antibody
    • T complex testis expressed3 antibody
    • Tctex 3 antibody
    • Tctex3 antibody
    see all

Images

  • All lanes : Anti-PHF1 antibody [EPR14222] (ab184951) at 1/20000 dilution

    Lane 1 : 293 lysate
    Lane 2 : Jurkat lysate
    Lane 3 : MCF7 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 62 kDa

  • Anti-PHF1 antibody [EPR14222] (ab184951) at 1/5000 dilution + Human fetal brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 62 kDa

  • Immunofluorescent analysis of MCF7 cells (4% paraformaldehyde-fixed) labeling PHF1 with ab184951 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Flow Cytometric analysis of 293 cells (2% paraformaldehyde-fixed) labeling PHF1 with ab184951 at 1/160 dilution (red) mpared to a negative control antibody (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling PHF1 with ab184951 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PHF1 with ab184951 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

References

This product has been referenced in:
  • Liu H  et al. Guanosine monophosphate reductase 1 is a potential therapeutic target for Alzheimer's disease. Sci Rep 8:2759 (2018). IHC ; Mouse . Read more (PubMed: 29426890) »
  • Nie SD  et al. High glucose forces a positive feedback loop connecting ErbB4 expression and mTOR/S6K pathway to aggravate the formation of tau hyperphosphorylation in differentiated SH-SY5Y cells. Neurobiol Aging 67:171-180 (2018). Read more (PubMed: 29674181) »

See all 5 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis)
Permeabilization
Yes - 0.1% Tween
Specification
Testis
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

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Verified customer

Submitted Mar 07 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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