Overview

  • Product nameAnti-PHF12 antibody
  • Description
    Rabbit polyclonal to PHF12
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 864-913 (VDNVLYSCDF SEKTPPTPPS SIVAKVQSVI RRRRHQKQDE EPSEEAAMMS) of Human PHF12 (NP_001028733).

  • Positive control
    • Human fetal brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab81249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 110 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

Target

  • RelevancePHF12 acts as a transcriptional repressor. It is involved in recruitment of functional SIN3A complexes to DNA. PHF12 represses transcription at least in part through the activity of an associated histone deacetylase (HDAC). It may also repress transcription in a SIN3A-independent manner through recruitment of functional AES complexes to DNA.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • FLJ34122 antibody
    • KIAA1523 antibody
    • MGC131914 antibody
    • Pf1 antibody
    • PHD factor 1 antibody
    • PHD finger protein 12 antibody
    • PHD zinc finger transcription factor antibody
    • PHF 12 antibody
    see all

Anti-PHF12 antibody images

  • Anti-PHF12 antibody (ab81249) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 110 kDa
    Observed band size : 110 kDa
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

References for Anti-PHF12 antibody (ab81249)

ab81249 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"