Anti-PHF16 antibody (ab129495)
Key features and details
- Rabbit polyclonal to PHF16
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PHF16 antibody -
Description
Rabbit polyclonal to PHF16 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 773-823 of Human PHF16 (NP_055550.1).
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Positive control
- HeLa, 293T and Jurkat whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab129495 was affinity purified using an epitope specific to PHF16 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 94 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 94 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. -
Tissue specificity
Ubiquitously expressed, with highest levels in placenta and uterus. -
Sequence similarities
Belongs to the JADE family.
Contains 1 PHD-type zinc finger. - Information by UniProt
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Database links
- Entrez Gene: 9767 Human
- Omim: 300618 Human
- SwissProt: Q92613 Human
- Unigene: 371977 Human
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Alternative names
- JADE 3 antibody
- Jade family PHD finger protein 3 antibody
- JADE3 antibody
see all
Images
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All lanes : Anti-PHF16 antibody (ab129495) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 94 kDa
Exposure time: 3 minutes -
Detection of PHF16 by Western Blot of Immunprecipitate.
ab129495 at 1 µg/ml staining PHF16 in HeLa whole cell lysate immunoprecipitated using ab129495 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: control IgG.
Predicted band size : 94 kDa.
Detection: Chemiluminescence with exposure time of 3 minutes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab129495 has been referenced in 1 publication.
- Pardo M et al. Myst2/Kat7 histone acetyltransferase interaction proteomics reveals tumour-suppressor Niam as a novel binding partner in embryonic stem cells. Sci Rep 7:8157 (2017). PubMed: 28811661