The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 96 kDa).
Use a concentration of 10 µg/ml. Requires purification with Protein A prior to ICC/IF.
FunctionComponent of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Transcriptional coactivator it may also promote acetylation of nucleosomal histone H4 by KAT5. Promotes apoptosis. May act as a renal tumor suppressor.
Tissue specificityHighly expressed in kidney. Also present in pancreas, liver and heart (at protein level). Down-regulated in renal cancer cells.
Sequence similaritiesBelongs to the JADE family. Contains 2 PHD-type zinc fingers.
DomainThe 2 PHD-type zinc fingers are required for transcriptional activity.