Overview

  • Product nameAnti-PHF19 antibody
    See all PHF19 primary antibodies
  • Description
    Mouse polyclonal to PHF19
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    PHF19 (NP_001009936.1, 1 a.a. ~ 207 a.a) full-length human protein.

  • Positive control
    • transfected 293T cell lysate; pancreas lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab68959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 65 kDa.

Target

  • FunctionActs as a transcritpional repressor. Isoform 1 and isoform 2 inhibit transcription from an HSV-tk promoter.
  • Tissue specificityIsoform 1 is expressed in thymus, heart, lung and kidney. Isoform 2 is predominantly expressed in placenta, skeletal muscle and kidney, whereas isoform 1 is predominantly expressed in liver and peripheral blood leukocytes. Overexpressed in many types of cancers, including colon, skin, lung, rectal, cervical, uterus, liver cancers, in cell lines derived from different stages of melanoma and in glioma cell lines.
  • Sequence similaritiesContains 2 PHD-type zinc fingers.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • hPCL3 antibody
    • MGC131698 antibody
    • MGC149712 antibody
    • MGC149713 antibody
    • MGC23929 antibody
    • MTF2L1 antibody
    • OTTHUMP00000021996 antibody
    • OTTHUMP00000021998 antibody
    • PCL3 antibody
    • PHD finger protein 19 antibody
    • Phf19 antibody
    • PHF19 protein antibody
    • PHF19_HUMAN antibody
    • Polycomb like 3 antibody
    • Polycomb like protein 3 antibody
    • Polycomb-like protein 3 antibody
    • TDRD19B antibody
    • Tudor domain containing 19B antibody
    see all

Anti-PHF19 antibody images

  • All lanes : Anti-PHF19 antibody (ab68959) at 1/500 dilution

    Lane 1 : transfected 293T cell lysate
    Lane 2 : non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 65 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence using ab68959 on HeLa cells [antibody concentration 10 ug/ml].

References for Anti-PHF19 antibody (ab68959)

This product has been referenced in:
  • Ghislin S  et al. PHF19 and Akt control the switch between proliferative and invasive states in melanoma. Cell Cycle 11:1634-45 (2012). Read more (PubMed: 22487681) »

See 1 Publication for this product

Product Wall

Thank you for contacting us. I have had a chance to speak with the lab regarding your question. They have found 3 isoforms of PHF19 protein all contain the immunogen a.a. sequence used to produce the ab68959.(http://www.uniprot.org/uniprot/Q5T6S3 )...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"