Overview

  • Product nameAnti-PHF19 antibody
    See all PHF19 primary antibodies
  • Description
    Rabbit polyclonal to PHF19
  • SpecificityDetects isoform 2.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 143-192 (FCRRCIFALA VRVSLPSSPV PASPASSSGA DQRLPSQSLS SKQKGHTWAL) of Human PHF19 (NP_001009936).

  • Positive control
    • HepG2 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab81166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 22 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay:1 /12500.

Target

  • FunctionActs as a transcritpional repressor. Isoform 1 and isoform 2 inhibit transcription from an HSV-tk promoter.
  • Tissue specificityIsoform 1 is expressed in thymus, heart, lung and kidney. Isoform 2 is predominantly expressed in placenta, skeletal muscle and kidney, whereas isoform 1 is predominantly expressed in liver and peripheral blood leukocytes. Overexpressed in many types of cancers, including colon, skin, lung, rectal, cervical, uterus, liver cancers, in cell lines derived from different stages of melanoma and in glioma cell lines.
  • Sequence similaritiesContains 2 PHD-type zinc fingers.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • hPCL3 antibody
    • MGC131698 antibody
    • MGC149712 antibody
    • MGC149713 antibody
    • MGC23929 antibody
    • MTF2L1 antibody
    • OTTHUMP00000021996 antibody
    • OTTHUMP00000021998 antibody
    • PCL3 antibody
    • PHD finger protein 19 antibody
    • Phf19 antibody
    • PHF19 protein antibody
    • PHF19_HUMAN antibody
    • Polycomb like 3 antibody
    • Polycomb like protein 3 antibody
    • Polycomb-like protein 3 antibody
    • TDRD19B antibody
    • Tudor domain containing 19B antibody
    see all

Anti-PHF19 antibody images

  • Anti-PHF19 antibody (ab81166) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 22 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

References for Anti-PHF19 antibody (ab81166)

ab81166 has not yet been referenced specifically in any publications.

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