The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: Use at 5-15µg/mg of lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 115 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPossible transcription factor.
Tissue specificityExpressed in heart, kidney, liver, lung, pancreas, placenta, spleen and testis. Not expressed in brain, skeletal muscle, colon, ovary, prostate, small intestine and thymus. Expressed in colon and ovary cancer cell lines while it is not expressed in the respective normal tissues.
All lanes : Anti-PHF20 antibody (ab91434) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg
developed using the ECL technique
Predicted band size : 115 kDa
Exposure time : 3 minutes
Immunoprecipitation - PHF20 antibody (ab91434)
1mg whole cell lysate from HeLa cells was immunoprecipitated using 10µg/mg ab91434. 20% IP was loaded per lane, and probed with ab91434 at 1µg/ml (lane 1) or a control IgG (lane 2). Detection: chemoluminescence with exposure time of 30 sec.
References for Anti-PHF20 antibody (ab91434)
has not yet been referenced specifically in any publications.
Publishing research using ab91434? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"