Overview

  • Product nameAnti-PHF20 antibody
    See all PHF20 primary antibodies
  • Description
    Rabbit polyclonal to PHF20
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Guinea pig, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide designed within residues:

    ETDHKSLSSSP DKREKFKEQR KATVNVKKDK EDKPLKTEKR PKQPDKEGKL

    , corresponding to amino acids 150-200 of Human PHF20 (NP_057520.2).

  • Positive control
    • HeLa cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab91434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 5-15µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 115 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPossible transcription factor.
    • Tissue specificityExpressed in heart, kidney, liver, lung, pancreas, placenta, spleen and testis. Not expressed in brain, skeletal muscle, colon, ovary, prostate, small intestine and thymus. Expressed in colon and ovary cancer cell lines while it is not expressed in the respective normal tissues.
    • Sequence similaritiesContains 1 A.T hook DNA-binding domain.
      Contains 1 C2H2-type zinc finger.
      Contains 1 PHD-type zinc finger.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • C20orf104 antibody
      • GLEA2 antibody
      • Glioma-expressed antigen 2 antibody
      • HCA58 antibody
      • Hepatocellular carcinoma-associated antigen 58 antibody
      • Novel zinc finger protein antibody
      • NZF antibody
      • PHD finger protein 20 antibody
      • PHF20 antibody
      • PHF20_HUMAN antibody
      • Transcription factor TZP antibody
      • TZP antibody
      see all

    Anti-PHF20 antibody images

    • All lanes : Anti-PHF20 antibody (ab91434) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 115 kDa


      Exposure time : 3 minutes
    • 1mg whole cell lysate from HeLa cells was immunoprecipitated using 10µg/mg ab91434.
      20% IP was loaded per lane, and probed with ab91434 at 1µg/ml (lane 1) or a control IgG (lane 2).
      Detection: chemoluminescence with exposure time of 30 sec.

    References for Anti-PHF20 antibody (ab91434)

    ab91434 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"