Overview

  • Product nameAnti-PHF20 antibody
    See all PHF20 primary antibodies
  • Description
    Rabbit polyclonal to PHF20
  • Tested applicationsSuitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 900-950 (PGSPKVKEYVS KKALPEEAPA RKLLDRGGEG LLSSQHQWQF NLLTHVESLQ) of Human PHF20 (NP_057520.2).

  • Positive control
    • HeLa cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab91456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 5-15 µg/mg of lysate.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionPossible transcription factor.
    • Tissue specificityExpressed in heart, kidney, liver, lung, pancreas, placenta, spleen and testis. Not expressed in brain, skeletal muscle, colon, ovary, prostate, small intestine and thymus. Expressed in colon and ovary cancer cell lines while it is not expressed in the respective normal tissues.
    • Sequence similaritiesContains 1 A.T hook DNA-binding domain.
      Contains 1 C2H2-type zinc finger.
      Contains 1 PHD-type zinc finger.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • C20orf104 antibody
      • GLEA2 antibody
      • Glioma-expressed antigen 2 antibody
      • HCA58 antibody
      • Hepatocellular carcinoma-associated antigen 58 antibody
      • Novel zinc finger protein antibody
      • NZF antibody
      • PHD finger protein 20 antibody
      • PHF20 antibody
      • PHF20_HUMAN antibody
      • Transcription factor TZP antibody
      • TZP antibody
      see all

    Anti-PHF20 antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling PHF20 with ab91456 at 1/1000 (1µg/ml). Detection: DAB.
    • Detection of Human PHF20 by Western Blot of Immunoprecipitates.
      Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded).
      ab91456 was used for IP at 10 µg/mg lysate (lane 1), with a control IgG in lane 2. For blotting immunoprecipitates, an anti-PHF20 was used at 1 µg/ml.
      Detection: Chemiluminescence with an exposure time of 30 seconds.

    References for Anti-PHF20 antibody (ab91456)

    ab91456 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"