The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 5-15 µg/mg of lysate.
Application notesIs unsuitable for WB.
FunctionPossible transcription factor.
Tissue specificityExpressed in heart, kidney, liver, lung, pancreas, placenta, spleen and testis. Not expressed in brain, skeletal muscle, colon, ovary, prostate, small intestine and thymus. Expressed in colon and ovary cancer cell lines while it is not expressed in the respective normal tissues.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling PHF20 with ab91456 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation - PHF20 antibody (ab91456)
Detection of Human PHF20 by Western Blot of Immunoprecipitates.
Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded).
ab91456 was used for IP at 10 µg/mg lysate (lane 1), with a control IgG in lane 2. For blotting immunoprecipitates, an anti-PHF20 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
References for Anti-PHF20 antibody (ab91456)
has not yet been referenced specifically in any publications.
Publishing research using ab91456? Please let us know so that we can cite the reference in this datasheet.
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