Overview

  • Product name
  • Description
    Mouse polyclonal to PHF20L1
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length PHF20L1 protein (Human)

  • Positive control
    • PHF20L1 transfected 293T cell lysate and Hela cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab67796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 46 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Sequence similarities
    Contains 1 PHD-type zinc finger.
    Contains 2 Tudor domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • CGI 72 antibody
    • MGC64923 antibody
    • OTTHUMP00000192870 antibody
    • OTTHUMP00000192871 antibody
    • OTTHUMP00000192872 antibody
    • OTTHUMP00000192874 antibody
    • OTTHUMP00000192875 antibody
    • OTTHUMP00000192876 antibody
    • OTTHUMP00000192877 antibody
    • OTTHUMP00000227156 antibody
    • P20L1_HUMAN antibody
    • PHD finger protein 20 like 1 antibody
    • PHD finger protein 20 like 1, isoform CRA_a antibody
    • PHD finger protein 20-like protein 1 antibody
    • PHF20L1 antibody
    • PHF20L1 protein antibody
    • PHF20L1 transcript variant 3, mRNA antibody
    • TDRD20B antibody
    • Tudor domain containing 20B antibody
    • tudor domain-containing protein PHF20L1 antibody
    • Uncharacterized protein PHF20L1 antibody
    see all

Images

  • All lanes : Anti-PHF20L1 antibody (ab67796) at 1/500 dilution

    Lane 1 : PHF20L1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 46 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • ab67796, at 10µg/ml, staining PHF20L1 in Hela cell

References

This product has been referenced in:
  • Estève PO  et al. Methyllysine reader plant homeodomain (PHD) finger protein 20-like 1 (PHF20L1) antagonizes DNA (cytosine-5) methyltransferase 1 (DNMT1) proteasomal degradation. J Biol Chem 289:8277-87 (2014). ICC/IF ; Human . Read more (PubMed: 24492612) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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