Anti-PHF22 antibody - Aminoterminal end (ab71396)

Overview

  • Product name
    Anti-PHF22 antibody - Aminoterminal end
    See all PHF22 primary antibodies
  • Description
    Rabbit polyclonal to PHF22 - Aminoterminal end
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the N-terminal region of human PHF22.

  • Positive control
    • Hela cell line lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: 1/1000.
    WB: 1/100 - 1/500. Detects bands of approximately 37 and 49 kDa (predicted molecular weight: 49 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      PHF22 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates 3' end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690) (Baillat et al., 2005 [PubMed 16239144]). [OMIM]
    • Cellular localization
      Nucleus
    • Database links
    • Alternative names
      • Hypothetical Nuclear Factor SBBI22 antibody
      • INT12 antibody
      • Integrator complex subunit 12 antibody
      • PHD finger protein 22 antibody
      • SBBI22 antibody
      see all

    Anti-PHF22 antibody - Aminoterminal end images

    • Anti-PHF22 antibody - Aminoterminal end (ab71396) at 1/60 dilution + Hela cell line lysate at 35 µg

      Predicted band size : 49 kDa
      Observed band size : 49 kDa
      Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-PHF22 antibody - Aminoterminal end (ab71396)

    ab71396 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab71396.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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