Overview

  • Product nameAnti-PHF8 antibody
    See all PHF8 primary antibodies
  • Description
    Rabbit polyclonal to PHF8
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 650 and 700 of human PHF8 (CAI41576.1).

  • Positive control
    • Human controls: Breast Carcinoma, Colon Carcinoma, Pancreatic Islet Cell Tumor, Prostate Carcinoma, Stomach Adenocarcinoma Mouse control: Teratoma

Properties

Applications

Our Abpromise guarantee covers the use of ab84779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.

Target

  • FunctionHistone lysine demethylase with selectivity for the di-and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes. Required for brain development, probably by regulating expression of neuron-specific genes. Only has activity toward H4K20Me1 when nucleosome is used as a substrate and when not histone octamer is used as substrate. May also have weak activity toward dimethylated H3 'Lys-36' (H3K36Me2), however, the relevance of this result remains unsure in vivo. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3.
  • Involvement in diseaseDefects in PHF8 are the cause of mental retardation syndromic X-linked Siderius type (MRXSSD) [MIM:300263]. A disorder characterized by mild to borderline mental retardation with or without cleft lip/cleft palate.
  • Sequence similaritiesBelongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
    Contains 1 JmjC domain.
    Contains 1 PHD-type zinc finger.
  • DomainThe PHD-type zinc finger mediates the binding to H3K4me3. Binding to H3K4me3 promotes its access to H3K9me2.
    The linker region is a critical determinant of demethylase specificity. It enables the active site of JmjC to reach the target H3K9me2 when the PHD-type zinc finger binds to H3K4me3.
  • Post-translational
    modifications
    Phosphorylation at Ser-69 and Ser-120 are required for dissociation from chromatin and accumulation of H4K20Me1 levels during prophase.
  • Cellular localizationNucleus. Nucleus > nucleolus. Recruited to H3K4me3 sites on chromatin during interphase. Dissociates from chromatin when cells enter mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • Histone lysine demethylase PHF8 antibody
    • JHDM1F antibody
    • Jumonji C domain containing histone demethylase 1F antibody
    • MRXSSD antibody
    • PHD finger protein 8 antibody
    • PHF8 antibody
    • PHF8_HUMAN antibody
    • ZNF422 antibody
    see all

Anti-PHF8 antibody images

  • ab84779, at a 1/100 dilution, staining PHF8 in formalin fixed, paraffin embedded human breast carcinoma by Immunohistochemistry using DAB staining.
  • ab84779, at a 1/100 dilution, staining PHF8 in formalin fixed, paraffin embedded mouse teratoma by Immunohistochemistry using DAB staining.

References for Anti-PHF8 antibody (ab84779)

ab84779 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"