Synthetic peptide conjugated to KLH derived from within residues 800 - 900 of Human PHF8.
(Peptide available as ab36067.)
Our Abpromise guarantee covers the use of ab36068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Chromatin was prepared from SupT1 (human T-cell leukemia cell line) nuclear lysate. Cross-linking (X-ChIP) with 1% formaldehyde for 10 minutes. ChIP was performed with ab36068 at 0.1 μg/μg chromatin. Positive control: Transcriptional start site (TSS). Negative control: cells treated with PHF8-specific shRNA. The immunoprecipitated DNA was quantified by real time PCR.