Overview

  • Product nameAnti-PHGDH antibody
    See all PHGDH primary antibodies
  • Description
    Rabbit polyclonal to PHGDH
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within internal sequence amino acids 209-397 of Human PHGDH (NP_006614).

  • Positive control
    • 293T, Raji, A431 and Jurkat whole cell lysates; Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab125865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • PathwayAmino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.
  • Involvement in diseaseDefects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency) [MIM:601815]. It is characterized by congenital microcephaly, psychomotor retardation, and seizures.
  • Sequence similaritiesBelongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 PGDH antibody
    • 3-PGDH antibody
    • 3-phosphoglycerate dehydrogenase antibody
    • 3PGDH antibody
    • D-3-phosphoglycerate dehydrogenase antibody
    • EC 1.1.1.95 antibody
    • Epididymis secretory protein Li 113 antibody
    • HEL S 113 antibody
    • NLS antibody
    • NLS1 antibody
    • PDG antibody
    • PGAD antibody
    • PGD antibody
    • PGDH antibody
    • PGDH3 antibody
    • Phgdh antibody
    • PHGDHD antibody
    • Phosphoglycerate dehydrogenase antibody
    • SERA antibody
    • SERA_HUMAN antibody
    see all

Anti-PHGDH antibody images

  • All lanes : Anti-PHGDH antibody (ab125865) at 1/10000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 57 kDa
  • ab125865, at 1/250, staining PHGDH in formalin fixed, paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.

References for Anti-PHGDH antibody (ab125865)

ab125865 has not yet been referenced specifically in any publications.

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