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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer pH: 7.00 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 78% PBS, 20% Glycerol, 1% BSA
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab125865 is purified by antigen-affinity chromatography.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000 - 1/20000. Predicted molecular weight: 57 kDa.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Pathway Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.
Involvement in disease Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency) [MIM:601815]. It is characterized by congenital microcephaly, psychomotor retardation, and seizures.
Sequence similarities Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
Information by UniProt
3 PGDH antibody
3-phosphoglycerate dehydrogenase antibody
Anti-PHGDH antibody images
Western blot - Anti-PHGDH antibody (ab125865)
All lanes : Anti-PHGDH antibody (ab125865) at 1/10000 dilution Lane 1 : A431 whole cell lysate Lane 2 : Raji whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size : 57 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHGDH antibody (ab125865)
ab125865, at 1/250, staining PHGDH in formalin fixed, paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.
References for Anti-PHGDH antibody (ab125865)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"