• Product name
  • Description
    Mouse monoclonal to PHGDH
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-P, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-534 of Human PHGDH

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab211365 as a replacement.



Our Abpromise guarantee covers the use of ab57030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 57 kDa.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


IP Use at an assay dependent concentration.


  • Pathway
    Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.
  • Involvement in disease
    Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency) [MIM:601815]. It is characterized by congenital microcephaly, psychomotor retardation, and seizures.
  • Sequence similarities
    Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 PGDH antibody
    • 3-PGDH antibody
    • 3-phosphoglycerate dehydrogenase antibody
    • 3PGDH antibody
    • D-3-phosphoglycerate dehydrogenase antibody
    • EC antibody
    • Epididymis secretory protein Li 113 antibody
    • HEL S 113 antibody
    • NLS antibody
    • NLS1 antibody
    • PDG antibody
    • PGAD antibody
    • PGD antibody
    • PGDH antibody
    • PGDH3 antibody
    • Phgdh antibody
    • PHGDHD antibody
    • Phosphoglycerate dehydrogenase antibody
    • SERA antibody
    • SERA_HUMAN antibody
    see all


  • PHGDH antibody (ab57030) used in immunofluorescence at 1-5ug/ml on HeLa cells.
  • PHGDH antibody (ab57030) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human lymph node.
  • PHGDH was immunoprecipitated using 0.5mg Jurkat whole cell extract, 10µg of Mouse monoclonal to PHGDH and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab57030.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
    Band: 57kDa; PHGDH.
  • PHGDH antibody (ab57030) at 1ug/ml + Jurkat cell lysate at 25ug/lane.
  • Overlay histogram showing HeLa cells stained with ab57030 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57030, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.


This product has been referenced in:
  • Kitazawa S  et al. Succinate dehydrogenase B-deficient cancer cells are highly sensitive to bromodomain and extra-terminal inhibitors. Oncotarget 8:28922-28938 (2017). WB . Read more (PubMed: 28423651) »
  • Qu J  et al. Phosphoglycerate mutase 1 regulates dNTP pool and promotes homologous recombination repair in cancer cells. J Cell Biol 216:409-424 (2017). Read more (PubMed: 28122957) »

See all 5 Publications for this product

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