Overview

  • Product nameAnti-PHGDH antibody
    See all PHGDH primary antibodies
  • Description
    Mouse monoclonal to PHGDH
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-P, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-534 of Human PHGDH

Properties

Applications

Our Abpromise guarantee covers the use of ab57030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 57 kDa.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.

Target

  • PathwayAmino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.
  • Involvement in diseaseDefects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency) [MIM:601815]. It is characterized by congenital microcephaly, psychomotor retardation, and seizures.
  • Sequence similaritiesBelongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 PGDH antibody
    • 3-PGDH antibody
    • 3-phosphoglycerate dehydrogenase antibody
    • 3PGDH antibody
    • D-3-phosphoglycerate dehydrogenase antibody
    • EC 1.1.1.95 antibody
    • Epididymis secretory protein Li 113 antibody
    • HEL S 113 antibody
    • NLS antibody
    • NLS1 antibody
    • PDG antibody
    • PGAD antibody
    • PGD antibody
    • PGDH antibody
    • PGDH3 antibody
    • Phgdh antibody
    • PHGDHD antibody
    • Phosphoglycerate dehydrogenase antibody
    • SERA antibody
    • SERA_HUMAN antibody
    see all

Anti-PHGDH antibody images

  • PHGDH antibody (ab57030) used in immunofluorescence at 1-5ug/ml on HeLa cells.
  • PHGDH antibody (ab57030) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human lymph node.
  • PHGDH was immunoprecipitated using 0.5mg Jurkat whole cell extract, 10µg of Mouse monoclonal to PHGDH and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab57030.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
    Band: 57kDa; PHGDH.


  • Predicted band size : 57 kDa
  • Overlay histogram showing HeLa cells stained with ab57030 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57030, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-PHGDH antibody (ab57030)

This product has been referenced in:
  • Kim SK  et al. Differential expression of enzymes associated with serine/glycine metabolism in different breast cancer subtypes. PLoS One 9:e101004 (2014). WB ; Human . Read more (PubMed: 24979213) »
  • Bollig-Fischer A  et al. Oncogene activation induces metabolic transformation resulting in insulin-independence in human breast cancer cells. PLoS One 6:e17959 (2011). WB ; Human . Read more (PubMed: 21437235) »

See all 2 Publications for this product

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