Pleckstrin homology domain interacting protein antibody
WD repeat containing protein 11 antibody
WD repeat protein 11 antibody
Anti-PHIP antibody images
Western blot - PHIP antibody (ab57522)
Western blot against tagged recombinant protein immunogen using ab57522 PHIP antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa
Flow Cytometry - Anti-PHIP antibody (ab57522)
Overlay histogram showing HeLa cells stained with ab57522 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57522, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
References for Anti-PHIP antibody (ab57522)
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