Anti-PHIP antibody (ab86244)
Key features and details
- Rabbit polyclonal to PHIP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-PHIP antibody
See all PHIP primary antibodies -
Description
Rabbit polyclonal to PHIP -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat -
Immunogen
Synthetic peptide corresponding to Human PHIP aa 1600-1700.
Database link: Q8WWQ0 -
Positive control
- Whole cell lysate from HeLa cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/2000 - 1/10000. Detects a band of approximately 230 kDa (predicted molecular weight: 207 kDa).
|
Notes |
---|
WB
1/2000 - 1/10000. Detects a band of approximately 230 kDa (predicted molecular weight: 207 kDa). |
Target
-
Relevance
PHIP contains two bromo domains and eight WD repeats. It may be involved in insulin signaling. It is expressed in myeloma and epidermoid carcinoma cell lines. -
Database links
- Entrez Gene: 55023 Human
- Entrez Gene: 83946 Mouse
- Entrez Gene: 315843 Rat
- Omim: 612870 Human
- SwissProt: Q8WWQ0 Human
- SwissProt: Q8VDD9 Mouse
-
Alternative names
- DCAF14 antibody
- DDB1 and CUL4 associated factor 14 antibody
- FLJ20705 antibody
see all
Images
-
All lanes : Anti-PHIP antibody (ab86244) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 207 kDa
Observed band size: 230 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab86244 has been referenced in 4 publications.
- Su W et al. circPHIP promotes oral squamous cell carcinoma progression by sponging miR-142-5p and regulating PHIP and ACTN4 expression. Mol Ther Nucleic Acids 23:185-199 (2021). PubMed: 33376626
- Townsend A et al. DCAF14 promotes stalled fork stability to maintain genome integrity. Cell Rep 34:108669 (2021). PubMed: 33503431
- Marenne G et al. Exome Sequencing Identifies Genes and Gene Sets Contributing to Severe Childhood Obesity, Linking PHIP Variants to Repressed POMC Transcription. Cell Metab 31:1107-1119.e12 (2020). PubMed: 32492392
- McLure KG et al. RVX-208, an inducer of ApoA-I in humans, is a BET bromodomain antagonist. PLoS One 8:e83190 (2013). PubMed: 24391744