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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/2000 - 1/10000. Detects a band of approximately 230 kDa (predicted molecular weight: 207 kDa).
Is unsuitable for IP.
Anti-PHIP antibody images
Western blot - PHIP antibody (ab86244)
All lanes :
Anti-PHIP antibody (ab86244) at 0.04 µg/mlLane 1 :
Whole cell lysate from HeLa cells at 50 µgLane 2 :
Whole cell lysate from HeLa cells at 15 µgLane 3 :
Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL techniquePredicted band size :
207 kDaObserved band size :
230 kDa (why is the actual band size different from the predicted?
)Exposure time :
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"