Overview

  • Product name
    Anti-PHIP antibody
  • Description
    Rabbit polyclonal to PHIP
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1600 and 1650 of Human PHIP (NP_060404.3).

  • Positive control
    • Whole cell lysate from HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 230 kDa (predicted molecular weight: 207 kDa).
  • Application notes
    Is unsuitable for IP.
  • Target

    • Relevance
      PHIP contains two bromo domains and eight WD repeats. It may be involved in insulin signaling. It is expressed in myeloma and epidermoid carcinoma cell lines.
    • Database links
    • Alternative names
      • DCAF14 antibody
      • DDB1 and CUL4 associated factor 14 antibody
      • FLJ20705 antibody
      • FLJ45918 antibody
      • IRS 1 PH domain binding protein antibody
      • IRS1 PH domain binding protein antibody
      • MGC90216 antibody
      • Ndrp antibody
      • Neuronal differentiation related protein antibody
      • PH interacting protein antibody
      • Pleckstrin homology domain interacting protein antibody
      • WD repeat containing protein 11 antibody
      • WD repeat protein 11 antibody
      • WDR11 antibody
      see all

    Images

    • All lanes : Anti-PHIP antibody (ab86244) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 50 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 5 µg

      Developed using the ECL technique

      Predicted band size : 207 kDa
      Observed band size : 230 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

    References

    This product has been referenced in:

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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