Recombinant
RabMAb

Anti-PHKG1 antibody [EPR14812] (ab194112)

Overview

  • Product name
    Anti-PHKG1 antibody [EPR14812]
  • Description
    Rabbit monoclonal [EPR14812] to PHKG1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PHKG1 aa 1-100. The exact sequence is proprietary.
    Database link: Q16816

  • Positive control
    • Human skeletal muscle, mouse and rat muscle lysates; Human skeletal muscle, Human hepatocellular carcinoma and rat kidney tissues; A673 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab194112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.

Target

  • Function
    Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. In vitro, phosphorylates PYGM, TNNI3, MAPT/TAU, GAP43 and NRGN/RC3.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Domain
    The two calmodulin-binding domains appear to act in concert to bind a single molecule of calmodulin and are pseudosubstrate/autoinhibitory domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • PHK gamma M antibody
    • PHK-gamma-M antibody
    • Phkg antibody
    • PHKG1 antibody
    • PHKG1_HUMAN antibody
    • PHKIN01 antibody
    • Phosphorylase b kinase gamma catalytic chain antibody
    • Phosphorylase b kinase gamma catalytic chain skeletal muscle/heart isoform antibody
    • phosphorylase b kinase gamma catalytic chain, skeletal muscle isoform antibody
    • phosphorylase kinase gamma antibody
    • phosphorylase kinase subunit gamma 1 antibody
    • Phosphorylase kinase subunit gamma-1 antibody
    • phosphorylase kinase, gamma 1 (muscle) antibody
    • Phosphorylase kinase, muscle, gamma-1 antibody
    • Serine/threonine-protein kinase PHKG1 antibody
    • skeletal muscle/heart isoform antibody
    see all

Images

  • Anti-PHKG1 antibody [EPR14812] (ab194112) at 1/20000 dilution + Human skeletal muscle lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 45 kDa
    Observed band size: 40 kDa (why is the actual band size different from the predicted?)

  • All lanes : Anti-PHKG1 antibody [EPR14812] (ab194112) at 1/1000 dilution

    Lane 1 : Mouse muscle lysate
    Lane 2 : Rat muscle lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa
    Observed band size: 40 kDa (why is the actual band size different from the predicted?)

  • Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling PHKG1 with ab194112 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling PHKG1 with ab194112 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling PHKG1 with ab194112 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized A673 cells labeling PHKG1 with ab194112 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor®488) secondary antibody (ab150077) at 1/400 dilution. Nuclear counter stain is DAPI (blue).

References

ab194112 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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