Overview

  • Product nameAnti-PHLDA1 antibody
    See all PHLDA1 primary antibodies
  • Description
    Mouse polyclonal to PHLDA1
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length PHLDA1 protein (Human)

  • Positive control
    • PHLDA1 transfected 293T cell lysate and Hela cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab67849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 30 kDa).

Target

  • FunctionSeems to be involved in regulation of apoptosis. May be involved in detachment-mediated programmed cell death. May mediate apoptosis during neuronal development. May be involved in regulation of anti-apoptotic effects of IGF1. May be involved in translational regulation.
  • Tissue specificityWidely expressed with highest levels in pancreas. Strongly expressed by benign melanocytic nevi, and progressively reduced expressed in primary and metastatic melanomas (at protein level).
  • Sequence similaritiesContains 1 PH domain.
  • Cellular localizationCytoplasm. Cytoplasmic vesicle. Nucleus, nucleolus. Colocalizes with intracellular vesicles.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis associated nuclear protein antibody
    • Apoptosis-associated nuclear protein antibody
    • DT1P1B11 antibody
    • MGC131738 antibody
    • PHLA1_HUMAN antibody
    • PHLDA1 antibody
    • PHRIP antibody
    • Pleckstrin homology like domain family A member 1 antibody
    • Pleckstrin homology-like domain family A member 1 antibody
    • PQ rich protein antibody
    • PQ-rich protein antibody
    • PQR protein antibody
    • Proline- and glutamine-rich protein antibody
    • Proline- and histidine-rich protein antibody
    • T cell death associated gene antibody
    • T CELL DEATH ASSOCIATED GENE 51 antibody
    • T-cell death-associated gene 51 protein antibody
    • Tdag antibody
    • TDAG51 antibody
    see all

Anti-PHLDA1 antibody images

  • All lanes : Anti-PHLDA1 antibody (ab67849) at 1/500 dilution

    Lane 1 : PHLDA1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 30 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • ab67849 at 10µg/ml staining PHLDA1 in Hela cells.

References for Anti-PHLDA1 antibody (ab67849)

ab67849 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293T cells)
Gel Running Conditions Reduced Denaturing (10% SDS PAGE gel)
Loading amount 25 µg
Specification HEK293T cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Yupen Chung

Verified customer

Submitted Jan 14 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"