Anti-PHLDA3 antibody [4B6] (ab81464)
Key features and details
- Mouse monoclonal [4B6] to PHLDA3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-PHLDA3 antibody [4B6]
See all PHLDA3 primary antibodies -
Description
Mouse monoclonal [4B6] to PHLDA3 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide: MTAAATATVL KEGVLEKRSG GLLQLWKRKR C, corresponding to N terminal amino acids 1-31 of human PHLDA3
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Positive control
- MDA-MB-468 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Purification notes
The antibody was produced from the hybridoma cultured in serum free medium and purified under mild conditions by propriety chromatography processes. The antibody is filter sterilized. -
Clonality
Monoclonal -
Clone number
4B6 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent dilution. Predicted molecular weight: 14 kDa.
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Notes |
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WB
Use at an assay dependent dilution. Predicted molecular weight: 14 kDa. |
Target
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Function
p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its directs transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May acts as a tumor suppressor. -
Tissue specificity
Widely expressed with lowest expression in liver and spleen. -
Sequence similarities
Belongs to the PHLDA3 family.
Contains 1 PH domain. -
Domain
The PH domain binds phosphoinositides with a broad specificity. It competes with the PH domain of AKT1 and directly interferes with AKT1 binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-triphosphate (PIP3), preventing AKT1 association to membrane lipids and subsequent activation of AKT1 signaling. -
Cellular localization
Cytoplasm. Membrane. - Information by UniProt
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Database links
- Entrez Gene: 23612 Human
- Entrez Gene: 27280 Mouse
- Entrez Gene: 363989 Rat
- Omim: 607054 Human
- SwissProt: Q9Y5J5 Human
- SwissProt: Q9WV95 Mouse
- SwissProt: Q5PQT7 Rat
- Unigene: 268557 Human
see all -
Alternative names
- PHLA3_HUMAN antibody
- PHLDA 3 antibody
- phlda3 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab81464 has been referenced in 2 publications.
- Wang P et al. Pleckstrin homology-like domain family A, member 3, a miR-19a-3p-regulated gene, suppresses tumor growth in osteosarcoma by downregulating the Akt pathway. Bioengineered 13:3993-4009 (2022). PubMed: 35112982
- Prudovsky I et al. Phosphatidylserine colocalizes with epichromatin in interphase nuclei and mitotic chromosomes. Nucleus 3:200-10 (2012). PubMed: 22555604