Overview

  • Product name
    Anti-PHLDA3 antibody [4B6]
    See all PHLDA3 primary antibodies
  • Description
    Mouse monoclonal [4B6] to PHLDA3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide: MTAAATATVL KEGVLEKRSG GLLQLWKRKR C, corresponding to N terminal amino acids 1-31 of human PHLDA3

  • Positive control
    • MDA-MB-468 cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    The antibody was produced from the hybridoma cultured in serum free medium and purified under mild conditions by propriety chromatography processes. The antibody is filter sterilized.
  • Clonality
    Monoclonal
  • Clone number
    4B6
  • Isotype
    IgG2b
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 14 kDa.

Target

  • Function
    p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its directs transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May acts as a tumor suppressor.
  • Tissue specificity
    Widely expressed with lowest expression in liver and spleen.
  • Sequence similarities
    Belongs to the PHLDA3 family.
    Contains 1 PH domain.
  • Domain
    The PH domain binds phosphoinositides with a broad specificity. It competes with the PH domain of AKT1 and directly interferes with AKT1 binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-triphosphate (PIP3), preventing AKT1 association to membrane lipids and subsequent activation of AKT1 signaling.
  • Cellular localization
    Cytoplasm. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • PHLA3_HUMAN antibody
    • PHLDA 3 antibody
    • phlda3 antibody
    • Pleckstrin homology like domain family A member 2 antibody
    • Pleckstrin homology like domain family A member 3 antibody
    • Pleckstrin homology-like domain family A member 3 antibody
    • TDAG51/Ipl homolog 1 antibody
    • TDAG51/Ipl homologue 1 antibody
    • TIH 1 antibody
    • TIH1 antibody
    see all

Images



  • Predicted band size : 14 kDa

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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