• FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab22822 was purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab22822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).


  • Functionp53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its directs transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May acts as a tumor suppressor.
  • Tissue specificityWidely expressed with lowest expression in liver and spleen.
  • Sequence similaritiesBelongs to the PHLDA3 family.
    Contains 1 PH domain.
  • DomainThe PH domain binds phosphoinositides with a broad specificity. It competes with the PH domain of AKT1 and directly interferes with AKT1 binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-triphosphate (PIP3), preventing AKT1 association to membrane lipids and subsequent activation of AKT1 signaling.
  • Cellular localizationCytoplasm. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • PHLA3_HUMAN antibody
    • PHLDA 3 antibody
    • phlda3 antibody
    • Pleckstrin homology like domain family A member 2 antibody
    • Pleckstrin homology like domain family A member 3 antibody
    • Pleckstrin homology-like domain family A member 3 antibody
    • TDAG51/Ipl homolog 1 antibody
    • TDAG51/Ipl homologue 1 antibody
    • TIH 1 antibody
    • TIH1 antibody
    see all

Anti-PHLDA3 antibody images

  • Lanes 1 - 2 : Anti-PHLDA3 antibody (ab22822) at 1 µg/ml
    Lane 3 : Anti-myc tag antibody at 1/1000 dilution

    Lane 1 : HEK293 lysate overexpressing Human PHLDA3 in RIPA buffer at 10 µg
    Lane 2 : Mock-transfected HEK293
    Lane 3 : HEK293 lysate overexpressing Human PHLDA3 in RIPA buffer at 10 µg

    developed using the ECL technique

    Predicted band size : 14 kDa

References for Anti-PHLDA3 antibody (ab22822)

This product has been referenced in:
  • Ohki R  et al. PHLDA3 is a novel tumor suppressor of pancreatic neuroendocrine tumors. Proc Natl Acad Sci U S A 111:E2404-13 (2014). Read more (PubMed: 24912192) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MDA-MB-468 cells)
Loading amount 20 µg
Specification MDA-MB-468 cells
Gel Running Conditions Reduced Denaturing (15 %)
Blocking step Blocking One (Nakalai) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100%

Rieko Ohki

Verified customer

Submitted Apr 14 2009