Overview

  • Product name
  • Description
    Rabbit polyclonal to PHLPP
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide: DNREALYGVF DGDRNVEVPY LLQCTMSDIL AEELQKTKNE EEYMVNTFIV M, corresponding to amino acids 1200-1250 of Human PHLPP

  • Positive control
    • Y79 cell extract

Properties

Applications

Our Abpromise guarantee covers the use of ab135957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 185 kDa.

Target

  • Function
    Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and suppression of tumor growth. Controls the phosphorylation of AKT2 and AKT3 more efficiently than that of AKT1. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor. May act as a negative regulator of K-Ras signaling in membrane rafts.
  • Tissue specificity
    In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 78% of tested tumors (at protein level). Ubiquitously expressed in non-cancerous tissues.
  • Sequence similarities
    Contains 21 LRR (leucine-rich) repeats.
    Contains 1 PH domain.
    Contains 1 PP2C-like domain.
  • Domain
    The PH domain is required for interaction with PRKCB and its dephosphorylation.
  • Cellular localization
    Cytoplasm. Membrane. Nucleus. In colorectal cancer tissue, expression is concentrated at the lateral membrane of epithelial cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • hSCOP antibody
    • KIAA0606 antibody
    • PH domain and leucine rich repeat protein phosphatase antibody
    • PH domain leucine rich repeat containing protein phosphatase antibody
    • PH domain leucine rich repeat protein phosphatase antibody
    • PH domain leucine-rich repeat-containing protein phosphatase 1 antibody
    • PH domain-containing family E member 1 antibody
    • PHLP1_HUMAN antibody
    • PHLPP antibody
    • Phlpp1 antibody
    • Pleckstrin homology domain containing family E (with leucine rich repeats) member 1 antibody
    • Pleckstrin homology domain containing family E protein 1 antibody
    • Pleckstrin homology domain-containing family E member 1 antibody
    • PLEKHE 1 protein antibody
    • PLEKHE1 antibody
    • SCN circadian oscillatory protein antibody
    • SCOP antibody
    • Suprachiasmatic nucleus circadian oscillatory protein antibody
    • Suprachiasmatic nucleus circadian protein antibody
    see all

Images

  • Anti-PHLPP antibody (ab135957) at 1/500 dilution + Y79 cell extract at 30 µg

    Predicted band size : 185 kDa

References

ab135957 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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