Anti-PHLPP antibody - Aminoterminal end (ab71277)

Overview

  • Product name
    Anti-PHLPP antibody - Aminoterminal end
    See all PHLPP primary antibodies
  • Description
    Rabbit polyclonal to PHLPP - Aminoterminal end
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human PHLPP (N terminal) conjugated to keyhole limpet haemocyanin.

  • Positive control
    • K562 cell line lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab71277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Detects a band of approximately 72, 185 kDa (predicted molecular weight: 185 kDa).
ELISA 1/1000.

Target

  • Function
    Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and suppression of tumor growth. Controls the phosphorylation of AKT2 and AKT3 more efficiently than that of AKT1. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor. May act as a negative regulator of K-Ras signaling in membrane rafts.
  • Tissue specificity
    In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 78% of tested tumors (at protein level). Ubiquitously expressed in non-cancerous tissues.
  • Sequence similarities
    Contains 21 LRR (leucine-rich) repeats.
    Contains 1 PH domain.
    Contains 1 PP2C-like domain.
  • Domain
    The PH domain is required for interaction with PRKCB and its dephosphorylation.
  • Cellular localization
    Cytoplasm. Membrane. Nucleus. In colorectal cancer tissue, expression is concentrated at the lateral membrane of epithelial cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • hSCOP antibody
    • KIAA0606 antibody
    • PH domain and leucine rich repeat protein phosphatase antibody
    • PH domain leucine rich repeat containing protein phosphatase antibody
    • PH domain leucine rich repeat protein phosphatase antibody
    • PH domain leucine-rich repeat-containing protein phosphatase 1 antibody
    • PH domain-containing family E member 1 antibody
    • PHLP1_HUMAN antibody
    • PHLPP antibody
    • Phlpp1 antibody
    • Pleckstrin homology domain containing family E (with leucine rich repeats) member 1 antibody
    • Pleckstrin homology domain containing family E protein 1 antibody
    • Pleckstrin homology domain-containing family E member 1 antibody
    • PLEKHE 1 protein antibody
    • PLEKHE1 antibody
    • SCN circadian oscillatory protein antibody
    • SCOP antibody
    • Suprachiasmatic nucleus circadian oscillatory protein antibody
    • Suprachiasmatic nucleus circadian protein antibody
    see all

Anti-PHLPP antibody - Aminoterminal end images

  • Anti-PHLPP antibody - Aminoterminal end (ab71277) at 1/60 dilution + K562 cell line lysate at 35 µg

    Predicted band size : 185 kDa
    Observed band size : 185 kDa
    Additional bands at : 72 kDa. We are unsure as to the identity of these extra bands.

References for Anti-PHLPP antibody - Aminoterminal end (ab71277)

ab71277 has not yet been referenced specifically in any publications.

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