Overview

  • Product nameAnti-PHLPP2 antibody
    See all PHLPP2 primary antibodies
  • Description
    Rabbit polyclonal to PHLPP2
  • Tested applicationsSuitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Guinea pig, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1275 and the C-terminus (residue 1323) of human PHLPP2 (NP_055835.2)

  • Positive control
    • Whole cell lysates from HeLa, 293T or NIH/3T3 cells. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: A549

Properties

Applications

Our Abpromise guarantee covers the use of ab71973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/2000 - 1/10000. Detects a band of approximately 190 kDa (predicted molecular weight: 147 kDa).
IP Use at 1-4 µg/mg of lysate.

Target

  • FunctionProtein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor.
  • Tissue specificityIn colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 80% of tested tumors (at protein level).
  • Sequence similaritiesContains 22 LRR (leucine-rich) repeats.
    Contains 1 PH domain.
    Contains 1 PP2C-like domain.
  • Cellular localizationCytoplasm. Membrane. Nucleus. In colorectal cancer tissue, expression is concentrated in the cytoplasm and nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0931 antibody
    • PH domain and leucine rich repeat protein phosphatase 2 antibody
    • PH domain leucine rich repeat protein phosphatase like antibody
    • PH domain leucine-rich repeat-containing protein phosphatase 2 antibody
    • PH domain leucine-rich repeat-containing protein phosphatase-like antibody
    • PHLP2_HUMAN antibody
    • PHLPP like antibody
    • PHLPP-like antibody
    • Phlpp2 antibody
    • PHLPPL antibody
    see all

Anti-PHLPP2 antibody images

  • All lanes : Anti-PHLPP2 antibody (ab71973) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 147 kDa
    Observed band size : 190 kDa (why is the actual band size different from the predicted?)
  • Detection of PHLPP2 by Western Blot of Immunoprecipitate.
    ab71973 at 1µg/ml staining PHLPP2 in HeLa whole cell lysate immunoprecipitated using ab71973 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: chemiluminescence with exposure time of 30 seconds.
  • ab71973 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab71973 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-PHLPP2 antibody (ab71973)

This product has been referenced in:
  • Liao WT  et al. microRNA-224 promotes cell proliferation and tumor growth in human colorectal cancer by repressing PHLPP1 and PHLPP2. Clin Cancer Res 19:4662-72 (2013). Read more (PubMed: 23846336) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"