Overview

  • Product namePhorbol 12-myristate 13-acetate (PMA)
  • Description
    PKC activator
  • Alternative names
    • PMA
  • Biological descriptionPotent nanomolar activator of protein kinase C in vivo and in vitro. Binds to C1 domain of protein kinase C, induces membrane translocation and enzyme activation. Also reported to have actions on non-kinase proteins including chimaerins, RasGRP and Unc-13/Munc-13. Extremely potent mouse skin tumor promoter.
  • Purity> 99%

Properties

  • Chemical namePhorbol 12-myristate 13-acetate
  • Molecular weight616.83
  • Chemical structureChemical Structure
  • Molecular formulaC36H56O8
  • CAS Number16561-29-8
  • PubChem identifier27924
  • Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overviewSoluble in DMSO to 100 mM and in ethanol to 10 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILESCC(=O)O[C@@]43[C@H](OC(=O)CCCCCCCCCCCCC)[C@@H](C)[C@@]1(O)[C@@H](C=C(CO)C[C@]2(O)C(=O)C(C)=C[C@@H]12)[C@@H]4C3(C)C
  • Source

    Synthetic

  • Research areas

    Associated products

    Applications

    Our Abpromise guarantee covers the use of ab120297 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Phorbol 12-myristate 13-acetate (PMA) images

    • Serum starved HeLa cells were incubated at 37°C for 60 minutes with vehicle control (0 µM) and different concentrations of phorbol 12-myristate 13-acetate (PMA) (ab120297) in DMSO. Increased expression of PKC mu (phospho S916) (ab81218) correlates with an increase in phorbol 12-myristate 13-acetate (PMA) concentration, as described in literature.

      Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab81218 at 1 µg/ml and ab8227 at 1 µg /ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

    • Sandwich ELISA - TNF alpha Human ELISA Kit (ab100654)

      TNFa detected in supernatants from control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) (P), and PMA with the addition of 1 ug x mL-1 of LPS (Sigma) (P+L) for the last 6 hours. PBMCs were stimulated for 48 hours with 2 % PHA-M (LifeTechnologies). Results shown after background signal was subtracted (duplicates +/- SD).

    • Sandwich ELISA - IL-6 (Interleukin-6) Mouse ELISA Kit (ab100712).

      IL-6 detected in supernatants from RAW 246.7 control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) (P), or 24 hours with PMA and 1 ug x mL-1 of LPS (Sigma) (P+L) for the last 6 hours. Results shown after background signal was subtracted (duplicates +/- SD).

    • Sandwich ELISA - IL-1ra (Interleukin-1ra) Mouse ELISA Kit (ab113348)

      IL-1Ra detected in supernatants from RAW 246.7 control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) (P), or 24 hours with PMA and 1 ug x mL-1 of LPS (Sigma) (P+L) for the last 6 hours. Results shown after background signal was subtracted (duplicates +/- SD).

    • Sandwich ELISA - IFN gamma Human ELISA Kit (ab46025)

      Jurkat were stimulated for 48 hours with 50 ng x mL-1 of PMA (ab120297) and 1 µM Ionomycin (ab120116) and PBMCs were stimulated for 48 hours with 2 % PHA-M (LifeTechnologies). Cell free supernatants were tested, showing results after background signal was subtracted (duplicates +/- SD).

    References for Phorbol 12-myristate 13-acetate (PMA) (ab120297)

    This product has been referenced in:
    • Kazanietz MG Eyes wide shut: protein kinase C isozymes are not the only receptors for the phorbol ester tumor promoters. Mol Carcinog 28:5-11 (2000). Read more (PubMed: 10820483) »
    • Kazanietz MG  et al. Pharmacology of the receptors for the phorbol ester tumor promoters: multiple receptors with different biochemical properties. Biochem Pharmacol 60:1417-24 (2000). Read more (PubMed: 11020443) »
    • Ryves WJ  et al. Activation of the PKC-isotypes alpha, beta 1, gamma, delta and epsilon by phorbol esters of different biological activities. FEBS Lett 288:5-9 (1991). Read more (PubMed: 1879564) »

    See all 4 Publications for this product

    Product Wall

    I can confirm that PMA is indeed Phorbol 12-myristate 13-acetate and it was used to induce the modification of Phospho-Smad2 at S467 site.

    Thank you for contacting us.

    We have not specifically tested ab52147 on C2C12 cells. However, we do have customer feedback regarding the use of this antibody to detect the phosphorylated protein in treated myometrial smooth muscle cells. Read More

    Thank you for contacting us.

    Our productab120297 Phorbol 12-myristate 13-acetate is 99% has a purity. We do recommend always sterile filtering any reagent for use in cell culture work.

    I hope this information is helpful to yo...

    Read More

    Vielen Dank für Ihre Anfrage und dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.

    Die Bande bei 50 kDa sieht in der Tat unspezifisch aus; dies wäre allerdings ja nicht weiter schlimm, wenn Sie die spezifische Ban...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"