Overview

  • Product namePhosphate Assay Kit (Fluorometric)
    See all Phosphate kits
  • Tests
    100 x 1 assay
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate
  • Assay typeQuantitative
  • Sensitivity
    > 0.1 nmol/well
  • Range
    2 µM - 10 µM
  • Assay time
    2h 00m
  • Product overview

    Abcam's Phosphate Assay Kit (Fluorometric) provides a sensitive, easy, quick means of assessing phosphate over a wide range of concentrations. In the assay, inorganic phosphate reacted with maltose to produce glucose in the present of a special enzyme. The glucose is specifically oxidized to generate a product that reacts with the OxiRed probe to generate fluorescence (Ex/Em=535/587nm). The kit can be used to detect Pi produced through reactions involving ATPases, GTPases, 5'-nucleotidase, protein phosphatases, acid and alkaline phosphatases, and phosphorylase, etc. from a variety of samples. Glucose background can be eliminated by doing a glucose control.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Inorganic phosphate (Pi) is one of the most important ions in biological systems. It functions in a variety of roles. One of the most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. A highly sensitive assay is desired to monitor Pi in variety samples or monitor Pi changes during kinase and phosphatase reactions.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Converter Blue 1 vial
    Developer Green 1 vial
    Phosphate Assay Buffer WM 1 x 25ml
    Phosphate Standard Yellow 1 x 50µl
    Phosphate Substrate Purple 1 x 0.2ml
    PicoProbe™ (in DMSO) Blue 1 x 200µl
  • Research areas
  • RelevancePhosphate is one of the most important of the inorganic ions in biological systems. It functions in a variety of roles. One of the most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. Phosphate is also of great importance in mineralization processes and is a primary stimulus of algal blooms frequently found in bodies of fresh water, due to run-off from areas of high fertilizer use.

Applications

Our Abpromise guarantee covers the use of ab102508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Phosphate Assay Kit (Fluorometric) images

  • Phosphate Standard Curve - 2 hours

Protocols

References for Phosphate Assay Kit (Fluorometric) (ab102508)

This product has been referenced in:
  • Dai H  et al. Transient binding of an activator BH3 domain to the Bak BH3-binding groove initiates Bak oligomerization. J Cell Biol 194:39-48 (2011). Read more (PubMed: 21727192) »

See 1 Publication for this product

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The laboratory have suggested the following protocol for the sample preparation:

Trypsinize cells and collect. Remove medium + Trypsin.

Add 2-3 volumes of cold assay buffer (200-300 ul buffer for 1...

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ab65622 is a colorimetric assay with a working range of 1-10uM concentration of phosphate. ab65622 provides enough materials to carry out 500 assays.

ab102508 is a more sensitive fluorometric...

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I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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The phosphate assay buffer, probe, converter and developer does not contain any known free interfering phosphate ions, if they does then blank control is to be used anyway, the reading of which will be deduced as backg...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"