Phosphatidylcholine Assay Kit (Colorimetric/Fluorometric) (ab83377) is a simple convenient means of measuring phosphatidylcholine in a variety of biological samples. This assay uses an enzyme-coupled reaction to hydrolyze phosphatidylcholine to release choline, which subsequently oxidizes the OxiRed probe in order to generate fluorescence (Ex/Em 535 nm 587 nm) and absorbance (570 nm). This assay measures phosphatidylcholine in the range of 0.1 to 10 nmol per sample.
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Phosphatidylcholine (PC) is a phospholipid which incorporates choline as the headgroup of the lipid. PC is a major constituent of biological membranes and is involved in cell signaling through release of choline by phospholipase D leaving the second messenger phosphatidic acid.
PC is present in serum at ~0.2-2.5 mM (~50-200 mg/dL).
|OxiRed Probe in DMSO||Red||1 x 200µl|
|PC Assay Buffer||WM||1 x 25ml|
|PC Development Mix (Lyophilised)||Green||1 vial|
|PC Hydrolysis Enzyme (lyophilized)||Purple||1 vial|
|PC Standard (10 µmol) (Lyophilised)||Yellow||1 vial|
Our Abpromise guarantee covers the use of ab83377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Phosphatidycholine levels measured fluorometrically in mouse tissue lysates (mg of extracted protein; background signal subtracted, mean of duplicates; +/- SD).
Phosphatidycholine levels colorimetrically measured in cell lysates (background signal subtracted, mean of duplicates; +/- SD).
Phosphatidycholine levels colorimetrically measured in rat biological fluids (background signal subtracted, mean of duplicates; +/- SD).