The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 21 kDa.
Staining of the Phosphinotricin Acetyl Transferase band by immunoblotting is specifically inhibited with the Phosphinotricin Acetyl Transferase immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceThe most commonly used selection markers in plant transformation are the bacterial bar gene encoding for the phosphinotricin acetyl transferase (PAT) and the gene encoding for neomycin phosphotransferase II (NPTII). The bar/pat gene was isolated from Streptomyces hygroscopicus. It encodes a small (21 kDa) protein. The pat gene confers resistance to the glufosinate ammonium herbicides bialaphos (BASTA), and phosphinotricin (PPT). PAT belongs to the family of acetyltransferases. It acetylates and inactivates the herbicides bialaphos and PPT. PAT can be used to detect relative transfection efficiencies in cells where multiple transfections are performed in parallel with different plasmids. PAT has been used routinely as a resistance gene in the production of genetically engineered crops and for the production of herbicide resistant crops. The PAT gene is an approved food additive for use as a processing aid in the development of new varieties of crops, including wheat, corn, and rice.
Phosphinothricin resistance protein antibody
PPT N acetyltransferase antibody
References for Anti-Phosphinotricin Acetyl Transferase antibody - Aminoterminal end (ab50504)
has not yet been referenced specifically in any publications.
Publishing research using ab50504? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"