Anti-Phosphinotricin Acetyl Transferase antibody - Aminoterminal end (ab50504)

Overview

  • Product nameAnti-Phosphinotricin Acetyl Transferase antibody - Aminoterminal end
  • Description
    Rabbit polyclonal to Phosphinotricin Acetyl Transferase - Aminoterminal end
  • SpecificityAb50504 (N terminal) recognizes bacterial Phosphinotricin Acetyl Transferase expressed in transgenic tobacco plants.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with Streptomyces hygroscopicus. Not yet tested in other species.
  • Immunogen

    Synthetic peptide:

    MSPERRPADIRRATEAD

    , corresponding to N terminal amino acids 1-17 of Streptomyces hygroscopicus Phosphinotricin Acetyl Transferase conjugated to KLH.

  • Positive control
    • Extract (cytosolic fraction) of leaves from transgenic Nicotiana tabacum expressing the Phosphinotricin Acetyl Transferase gene for Streptomyces hygroscopicus.

Properties

Applications

Our Abpromise guarantee covers the use of ab50504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 21 kDa.
    Staining of the Phosphinotricin Acetyl Transferase band by immunoblotting is specifically inhibited with the Phosphinotricin Acetyl Transferase immunizing peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe most commonly used selection markers in plant transformation are the bacterial bar gene encoding for the phosphinotricin acetyl transferase (PAT) and the gene encoding for neomycin phosphotransferase II (NPTII). The bar/pat gene was isolated from Streptomyces hygroscopicus. It encodes a small (21 kDa) protein. The pat gene confers resistance to the glufosinate ammonium herbicides bialaphos (BASTA), and phosphinotricin (PPT). PAT belongs to the family of acetyltransferases. It acetylates and inactivates the herbicides bialaphos and PPT. PAT can be used to detect relative transfection efficiencies in cells where multiple transfections are performed in parallel with different plasmids. PAT has been used routinely as a resistance gene in the production of genetically engineered crops and for the production of herbicide resistant crops. The PAT gene is an approved food additive for use as a processing aid in the development of new varieties of crops, including wheat, corn, and rice.
    • Alternative names
      • PAT antibody
      • Phosphinothricin resistance protein antibody
      • PPT N acetyltransferase antibody

    References for Anti-Phosphinotricin Acetyl Transferase antibody - Aminoterminal end (ab50504)

    ab50504 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"