Anti-Phospho S6K Peptide Substrate antibody (ab9973)

Overview

  • Product nameAnti-Phospho S6K Peptide Substrate antibody
    See all Phospho S6K Peptide Substrate primary antibodies
  • Description
    Rabbit polyclonal to Phospho S6K Peptide Substrate
  • SpecificitySpecifically reacts with phosphorylated S6 peptide substrate. Does not cross-react with the non-phosphorylated substrate peptide.
  • Tested applicationsSuitable for: WB, Othermore details
  • Immunogen

    KLH-phospho-S6 peptide (KRRRLApSLR) conjugates.

  • Positive control
    • Biotinylated S6 peptide phosphorylated with S6K or PKC.
  • General notes


    Antigen is based on the versatile phospho-S6 substrate peptide(KRRRLApSLR). The S6 substrate peptide (KRRRLASLR) is well documented as a substrate for p70 S6K and other kinases such as PKC, PKM and PKA.

Properties

Applications

Our Abpromise guarantee covers the use of ab9973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 mg/ml.
Other Use at an assay dependent concentration.

Immuno-capture assay of kinase: Use immobilized at 5 mg/mL in carbonate buffer (pH8.5-9.0).

Anti-Phospho S6K Peptide Substrate antibody images

  • Sensitivity test of anti-pS6 HRP with both phosphorylated and non-phosphorylated S6 peptide substrate.

References for Anti-Phospho S6K Peptide Substrate antibody (ab9973)

ab9973 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry and patience. I'm afraid that all the information that I was able to obttain regarding your question is the following: 1. Biotinylated S6 peptide is phosphorylated by S6K in homogenous solution. 2. Then p70 S6K kinase reacti...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"