Anti-Phospho S6K Peptide Substrate antibody (HRP) (ab9974)

Overview

  • Product name
    Anti-Phospho S6K Peptide Substrate antibody (HRP)
    See all Phospho S6K Peptide Substrate primary antibodies
  • Description
    Rabbit polyclonal to Phospho S6K Peptide Substrate (HRP)
  • Conjugation
    HRP
  • Specificity
    Specifically reacts with phosphorylated S6 peptide substrate. Does not cross-react with the non-phosphorylated substrate peptide.
  • Tested applications
    Suitable for: Other, WBmore details
  • Immunogen

    KLH-phospho-S6 peptide (KRRRLApSLR) conjugates

  • Positive control
    • Biotinylated S6 peptide phosphorylated with S6K or PKC.
  • General notes

    Antigen is based on the versatile phospho-S6 substrate peptide(KRRRLApSLR). The S6 substrate peptide (KRRRLASLR) is well documented as a substrate for p70 S6K and other kinases such as PKC, PKM and PKA.

Properties

Applications

Our Abpromise guarantee covers the use of ab9974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Other Use at an assay dependent concentration.

Immuno-capture assay of kinase: Use immobilized at 5 mg/mL in carbonate buffer (pH8.5-9.0).

WB Use a concentration of 2 mg/ml.

Images

  • Sensitivity test of anti-pS6 HRP with both phosphorylated and non-phosphorylated S6 peptide substrate.

References

ab9974 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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