Anti-Phospho - (Ser/Thr) antibody (ab117253)
Key features and details
- Rabbit polyclonal to Phospho - (Ser/Thr)
- Suitable for: ELISA, Dot blot
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Phospho - (Ser/Thr) antibody -
Description
Rabbit polyclonal to Phospho - (Ser/Thr) -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, Dot blotmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Phosphoserine/threonine conjugated with R-PE.
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General notes
Buffers and proteins which contain phosphate should be avoided with this antibody. Certain proteins known to contain phosphorylated serine and threonine may not be detected by this antibody due to steric hindrance.
The immunogen used is Phosphoserine and phosphothreonine conjugated with R-PE.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.16% Tris HCl, 0.88% Sodium chloride -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117253 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
1/10000 - 1/50000.
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Dot blot |
Use at an assay dependent dilution.
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Notes |
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ELISA
1/10000 - 1/50000. |
Dot blot
Use at an assay dependent dilution. |
Target
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Relevance
A hallmark of signal transduction pathways is the reversible phosphorylation of serine and threonine residues within specific sequences, or motifs, in target proteins. Specific signaling motifs include not only sequences that are recognized by protein kinases, but also those that are recognized by phosphorylation-dependent binding proteins like 14-3-3. These modular phosphoprotein interacting domains are critical elements in modulating, directing and amplifying intracellular communications. Many critical protein kinases can be regulated by phosphorylation at a specific serine or threonine surrounded by phenylalanine or tyrosine. For example, Akt, an important kinase that regulates cell survival, is activated by phosphorylation at Ser473, a site surrounded by phenylalanine and tyrosine. RSK1, p70 S6 K, and certain PKC isoforms also contain a similar consensus phosphorylation site. Phosphorylation of these sites is required for kinase activity. -
Alternative names
- Phosphoserine antibody
- Phosphothreonine antibody
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (13)
ab117253 has been referenced in 13 publications.
- Shen Y et al. Methionine oxidation of CLK4 promotes the metabolic switch and redox homeostasis in esophageal carcinoma via inhibiting MITF selective autophagy. Clin Transl Med 12:e719 (2022). PubMed: 35092699
- Wang M et al. Blockade of phosphotyrosine pathways suggesting SH2 superbinder as a novel therapy for pulmonary fibrosis. Theranostics 12:4513-4535 (2022). PubMed: 35832075
- Wang Z et al. GlPP2C1 Silencing Increases the Content of Ganodermalingzhi Polysaccharide (GL-PS) and Enhances Slt2 Phosphorylation. J Fungi (Basel) 8:N/A (2022). PubMed: 36135674
- Lan C et al. Inhibition of DYRK1A, via histone modification, promotes cardiomyocyte cell cycle activation and cardiac repair after myocardial infarction. EBioMedicine 82:104139 (2022). IP ; Rat . PubMed: 35810562
- Xia C et al. Phosphatase OsPP2C27 directly dephosphorylates OsMAPK3 and OsbHLH002 to negatively regulate cold tolerance in rice. Plant Cell Environ 44:491-505 (2021). PubMed: 33150964