Anti-Phospho - (Ser/Thr) antibody (ab117253)

Overview

  • Product nameAnti-Phospho - (Ser/Thr) antibody
    See all Phospho - (Ser/Thr) primary antibodies
  • Description
    Rabbit polyclonal to Phospho - (Ser/Thr)
  • Specificityab117253 detects free and conjugated Phosphoserine/threonine as well as their modified cellular proteins. It does not cross-react with nonphosphorylated tyrosine, serine, threonine, AMP, or ATP.
  • Tested applicationsSuitable for: ELISA, Dot blotmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Phosphoserine/threonine conjugated with R-PE.

  • General notes

    Buffers and proteins which contain phosphate should be avoided with this antibody. Certain proteins known to contain phosphorylated serine and threonine may not be detected by this antibody due to steric hindrance.

    The immunogen used is Phosphoserine and phosphothreonine conjugated with R-PE.

     

     

Properties

Applications

Our Abpromise guarantee covers the use of ab117253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000 - 1/50000.
Dot blot Use at an assay dependent dilution.

Target

  • RelevanceA hallmark of signal transduction pathways is the reversible phosphorylation of serine and threonine residues within specific sequences, or motifs, in target proteins. Specific signaling motifs include not only sequences that are recognized by protein kinases, but also those that are recognized by phosphorylation-dependent binding proteins like 14-3-3. These modular phosphoprotein interacting domains are critical elements in modulating, directing and amplifying intracellular communications. Many critical protein kinases can be regulated by phosphorylation at a specific serine or threonine surrounded by phenylalanine or tyrosine. For example, Akt, an important kinase that regulates cell survival, is activated by phosphorylation at Ser473, a site surrounded by phenylalanine and tyrosine. RSK1, p70 S6 K, and certain PKC isoforms also contain a similar consensus phosphorylation site. Phosphorylation of these sites is required for kinase activity.
  • Alternative names
    • Phosphoserine antibody
    • Phosphothreonine antibody

References for Anti-Phospho - (Ser/Thr) antibody (ab117253)

ab117253 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Purified protein (recombinant protein)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 2 µg
Treatment Kinase domain of Obscurin
Specification recombinant protein
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
Username

Li Wang

Verified customer

Submitted Mar 25 2016

We do offer two products which may meet your needs, ab17464 and ab117253.

While not truly Pan phospho Ser/ThrAb14764 detects phosphoserine or phosphothreonine in the context of tyrosine, tryptophan or phenylalanine at the -1 position or phen...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"