Phosphoglucomutase Activity Assay Kit (Colorimetric) (ab155896)

Overview

  • Product name
    Phosphoglucomutase Activity Assay Kit (Colorimetric)
  • Sample type
    Plasma, Tissue, Adherent cells, Suspension cells, Tissue Homogenate
  • Assay type
    Enzyme activity
  • Sensitivity
    < 1 mU/well
  • Range
    2.5 nmol/well - 12.5 nmol/well
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mammal
  • Product overview

    In Abcam’s Phosphoglucomutase Activity Assay Kit (Colorimetric) (ab155896), PGM (phosphoglucomuyase) converts glucose-1-phosphate to glucose-6-phosphate; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm. The Phosphoglucomutase Assay Kit is simple, sensitive and rapid and can detect PGM activity even less than 1 mU/reaction.

  • Notes

    Phosphoglucomutase (PGM) plays a key role in carbohydrate metabolism and widely exists in all organisms. PGM interconverts Glucose- 1-Phosphate (G1P) and Glucose-6-Phosphate (G6P) depending on the body requirement. When glycogen breaks down, G1P is generated and phosphoglucomutase converts G1P to G6P, which can go either to glycolytic pathway to generate ATP, or to pentose phosphate pathway to generate ribose and NADPH. On the other hand, when cells have extra energy, PGM converts G6P to G1P, which generates glycogen. In humans, phosphoglucomutase have 2 isoforms (PGM I and PGM II). PGM deficiency leads to glucose storage disease. Detection of abnormal phosphoglucomutase activity is crucial for diagnosis, prediction and treatment of the disease.

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    NADH Standard Yellow 1 vial
    PGM Assay Buffer WM 1 x 27ml
    PGM Developer Red 1 vial
    PGM Enzyme Mix Green 1 vial
    PGM Positive Control Purple 1 vial
    PGM Substrate Blue 1 vial
  • Research areas

Images

  • Quantity of phosphoglucomutase in mouse serum. To control for background signal in samples, substrate was excluded in teh control. Measurements from samples (diluted 2 times) were taken after 10 and 20 mintues, the increase in signal was used to calculate the activity (duplicates; +/- SD).

  • Quantity of phosphoglucomutase in mouse kidney lysates, without prior ammonium sulfate precipitation. To control for background signal in samples, substrate was excluded in the control. The samples were tested in a range of different dilutions. Data shown is from 5 microgram kidney proteins. Measurements were taken after 10 and 20 mintues, the increase in signal was used to calculate the activity (duplicates; +/- SD).

  • Quantity of phosphoglucomutase in positive control and mouse liver and kidney lysates (without prior ammonium sulfate precipitation). The samples were tested in a range of different dilutions. Data shown is from 1 microgram of liver proteins (in 50 uL) and 5 microgram of kidney proteins. Meauserments were taken after 10 and 20 mintues, the increase in signal was used to calculate the activity after correction for the sample input (duplicates; +/- SD).

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Phosphoglucomutase activity in pure PGM Positive Control, jurkat cell lysate (induced with 2 µM camptothecin) and bovine liver lysate respectively

Protocols

References

ab155896 has not yet been referenced specifically in any publications.

Customer reviews and Q&As



The standard contains NADH only since this is what is measured as a read out (enzyme active then NAD zu NADH leading to colored product).

Please do not add anything to your samples:

There is no need to provide an exogenous...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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