Phosphoglucose Isomerase Activity Assay Kit (Colorimetric) (ab155897)

Overview

  • Product name
    Phosphoglucose Isomerase Activity Assay Kit (Colorimetric)
  • Sample type
    Tissue, Adherent cells, Suspension cells, Tissue Homogenate
  • Assay type
    Enzyme activity
  • Sensitivity
    < 0.1 mU/well
  • Range
    2.5 nmol/well - 12.5 nmol/well
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mammal
  • Product overview

    Abcam’s Phosphoglucose Isomerase Activity Assay Kit (Colorimetric) (ab155897) is an assay where PGI (phosphoglucose isomerase) converts fructose-6-phosphate to glucose-6-phosphate; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form a product, which reacts with a colorless probe to give strong absorbance at 450 nm. The PGI assay is simple, sensitive and rapid and can detect phosphoglucose isomerase activity less than 0.1 mU/reaction.

  • Notes

    Phosphoglucose isomerase (glucose-6-phosphate isomerase or PGI, EC 5.3.1.9) is an important housekeeping enzyme. PGI catalyzes the interconversion of glucose-6- phosphate to fructose-6-phosphate. PGI performs multiple functions & intracellularly plays key role in both glycolysis and gluconeogenesis. Extracellularly, PGI [also called Autocrine Motility Factor (AMF)] functions as a cytokine, which stimulates cell motility and is associated with tumor development and metastasis. In humans, PGI deficiency causes hemolytic anemia, whereas increased PGI activity is observed in many cancers such as gastrointestinal, kidney and breast cancer. Early detection of abnormal phosphoglucose isomerase activity is crucial for diagnosis, prediction and therapeutic strategy.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    NADH Standard Yellow 1 vial
    PGI Assay Buffer WM 1 x 27ml
    PGI Developer Red 1 vial
    PGI Enzyme Mix Green 1 vial
    PGI Positive Control Purple 1 vial
    PGI Substrate Blue 1 vial
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • Phosphoglucose Isomerase activity in bovine liver, HeLa cell lysate and positive control

Protocols

References

ab155897 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab155897.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up