Anti-Phospholamban antibody (ab126174)
Key features and details
- Rabbit polyclonal to Phospholamban
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Phospholamban antibody
See all Phospholamban primary antibodies -
Description
Rabbit polyclonal to Phospholamban -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Rabbit, Cow, Dog, Human, Pig -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-11 of Human Phospholamban (UniProt P26678).
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Positive control
- Mouse heart whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 6 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 6 kDa. |
Target
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Function
Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation. -
Tissue specificity
Heart muscle (at protein level). -
Involvement in disease
Cardiomyopathy, dilated 1P
Cardiomyopathy, familial hypertrophic 18 -
Sequence similarities
Belongs to the phospholamban family. -
Post-translational
modificationsPhosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes. -
Cellular localization
Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. Mitochondrion membrane. Membrane. Colocalizes with HAX1 at the endoplasmic reticulum (PubMed:17241641). Colocalizes with DMPK a the sarcoplasmic reticulum (PubMed:15598648). - Information by UniProt
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Database links
- Entrez Gene: 5350 Human
- Entrez Gene: 18821 Mouse
- Entrez Gene: 64672 Rat
- Omim: 172405 Human
- SwissProt: A4IFH6 Cow
- SwissProt: P61012 Dog
- SwissProt: P26678 Human
- SwissProt: P61014 Mouse
see all -
Alternative names
- Cardiac phospholamban antibody
- CMD1P antibody
- CMH18 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab126174 has been referenced in 1 publication.
- Quan C et al. SPEG Controls Calcium Re-Uptake into the Sarcoplasmic Reticulum Through Regulating SERCA2a by Its Second Kinase-Domain. Circ Res N/A:N/A (2018). PubMed: 30566039