Anti-Phospholamban antibody (ab86930)

Overview

  • Product nameAnti-Phospholamban antibody
    See all Phospholamban primary antibodies
  • Description
    Rabbit polyclonal to Phospholamban
  • SpecificityNo cross reactivity with other proteins is observed.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Phospholamban aa 2-16 (N terminal).
    Sequence:

    EKVQYLTRSAIRRAS


    Database link: P26678

  • Positive control
    • Rat heart tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab86930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 6 kDa.

Target

  • FunctionReversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation.
  • Tissue specificityHeart muscle (at protein level).
  • Involvement in diseaseCardiomyopathy, dilated 1P
    Cardiomyopathy, familial hypertrophic 18
  • Sequence similaritiesBelongs to the phospholamban family.
  • Post-translational
    modifications
    Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes.
  • Cellular localizationEndoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. Mitochondrion membrane. Membrane. Colocalizes with HAX1 at the endoplasmic reticulum (PubMed:17241641). Colocalizes with DMPK a the sarcoplasmic reticulum (PubMed:15598648).
  • Information by UniProt
  • Database links
  • Alternative names
    • Cardiac phospholamban antibody
    • CMD1P antibody
    • CMH18 antibody
    • PLB antibody
    • Pln antibody
    • PPLA_HUMAN antibody
    see all

Anti-Phospholamban antibody images

  • All lanes : Anti-Phospholamban antibody (ab86930) at 1 µg/ml

    Lane 1 : Rat heart tissue lysate
    Lane 2 : Rat heart tissue lysate


    Predicted band size : 6 kDa
    Observed band size : 11 kDa (why is the actual band size different from the predicted?)

References for Anti-Phospholamban antibody (ab86930)

ab86930 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. We have not tested these antibodies in guinea pig and therefore cannot guarantee they will work, however there is high homology between the immunogen and the guinea pig sequence for all three. Normally we predictlikely reac...

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Thank you for contacting Abcam.

I would be very happy to help answer your questions:

1 - What is the difference between ab86930 and ab85146? Which is more suitable for guinea pig?

- Both antibodies target different areas of t...

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