Anti-Phospholamban (phospho S16) antibody (ab15000)

Overview

  • Product nameAnti-Phospholamban (phospho S16) antibody
    See all Phospholamban primary antibodies
  • Description
    Rabbit polyclonal to Phospholamban (phospho S16)
  • SpecificityMultiple bands can be observed in addition to the 25kD pentamer for PLB. Monomeric, dimeric, trimeric forms, etc. can be observed if the preparations are boiled before SDS-PAGE. This antibody also can display non-specific bands at 75 and 100kD in some lysate preparations. Note: Do not boil cardiac preparation. Boiling may reduce the 25kD pentamer in to tri-, di- or monomers. In addition, phosphorylation may disrupt the pentamer into dimers and monomers.
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Hamster, Cow, Human, Pig, Chinese Hamster
    Predicted to work with: Dog
  • Immunogen

    KLH-conjugated, synthetic peptide corresponding to amino acids 14-25 of human cardiac phospholamban (

    RA[pS]TIEMPQQAR-C

    ) with an added C-terminal cysteine. The immunizing sequence is identical in mouse, rat, rabbit, pig and dog.

  • Positive control
    • Bovine cardiac preparation.

Properties

Applications

Our Abpromise guarantee covers the use of ab15000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 6.24 kDa.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionReversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation.
  • Tissue specificityHeart muscle (at protein level).
  • Involvement in diseaseCardiomyopathy, dilated 1P
    Cardiomyopathy, familial hypertrophic 18
  • Sequence similaritiesBelongs to the phospholamban family.
  • Post-translational
    modifications
    Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes.
  • Cellular localizationEndoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. Mitochondrion membrane. Membrane. Colocalizes with HAX1 at the endoplasmic reticulum (PubMed:17241641). Colocalizes with DMPK a the sarcoplasmic reticulum (PubMed:15598648).
  • Information by UniProt
  • Database links
  • Alternative names
    • Cardiac phospholamban antibody
    • CMD1P antibody
    • CMH18 antibody
    • PLB antibody
    • Pln antibody
    • PPLA_HUMAN antibody
    see all

Anti-Phospholamban (phospho S16) antibody images

  • All lanes : Anti-Phospholamban (phospho S16) antibody (ab15000) at 0.5 µg/ml

    Lane 1 : Bovine cardiac preparation treated with PKA
    Lane 2 : Untreated bovine cardiac preparation


    Predicted band size : 6.24 kDa
    Additional bands at : 14 kDa (possible multimer),23 kDa (possible multimer).
  • ab15000 staining Phospholamban (phospho S16) in Rat heart tissue by Immunohistochemistry (Frozen sections). The sections were fixed in Acetone prior to blocking with neat SuperBlock Blocking Buffer for 20 minutes at 23°C. The primary antibody was diluted 1/50 in PBS and incubated with the sample for 12 hours at 4°C. ab6720 - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Biotin) was used as the secondary antibody, diluted 1/100.

    See Abreview

References for Anti-Phospholamban (phospho S16) antibody (ab15000)

This product has been referenced in:
  • Tang Y  et al. Nitric oxide enhances extracellular ATP induced Ca²? oscillation in HeLa cells. Arch Biochem Biophys 565:68-75 (2015). Read more (PubMed: 25461674) »
  • Carneiro-Júnior MA  et al. The benefits of endurance training in cardiomyocyte function in hypertensive rats are reversed within four weeks of detraining. J Mol Cell Cardiol 57:119-28 (2013). WB ; Rat . Read more (PubMed: 23376037) »

See all 9 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Mouse Cell (myocyte)
Specification myocyte
Permeabilization No
Fixative Acetone
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Submitted Jun 17 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (15)
Sample Human Tissue lysate - whole (Heart)
Specification Heart
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jun 10 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Pig Tissue lysate - whole (Heart)
Loading amount 7.5 µg
Specification Heart
Gel Running Conditions Reduced Denaturing (14% PAGE)
Blocking step membrane blocking solution (Invitrogen) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
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Submitted Dec 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (heart)
Specification heart
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: universal decloaker
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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Submitted Nov 27 2012

Thank you for contacting us. We have not tested these antibodies in guinea pig and therefore cannot guarantee they will work, however there is high homology between the immunogen and the guinea pig sequence for all three. Normally we predictlikely reac...

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Thank you for contacting Abcam.

I would be very happy to help answer your questions:

1 - What is the difference between ab86930 and ab85146? Which is more suitable for guinea pig?

- Both antibodies target different areas of t...

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I am sorry to hear that you have been experiencing problems using this product in the application that you wish. Unfortunately, the attachment has not come through successfully. Could you please resend it to me? It is much appreciated.

In ord...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (heart)
Specification heart
Fixative Acetone
Permeabilization No
Blocking step SuperBlock Blocking Buffer as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 23°C
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Verified customer

Submitted Jan 19 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (heart tissue)
Loading amount 20 µg
Specification heart tissue
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Submitted Nov 04 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Chinese Hamster Cell lysate - whole cell (CHO cell line)
Loading amount 20 µg
Specification CHO cell line
Gel Running Conditions Reduced Denaturing (15% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jun 17 2009

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"