Recombinant
RabMAb

Anti-Phospholamban (phospho S16) antibody [EPR1911] (ab92697)

Overview

  • Product name
    Anti-Phospholamban (phospho S16) antibody [EPR1911]
    See all Phospholamban primary antibodies
  • Description
    Rabbit monoclonal [EPR1911] to Phospholamban (phospho S16)
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding Serine 16 of Human Phospholamban.

  • Positive control
    • Fetal heart lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab92697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: 1/50.
    WB: 1/200,000 - 1/500,000. Detects a band of approximately 11 kDa (predicted molecular weight: 6 kDa).

    Is unsuitable for IHC-P.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation.
    • Tissue specificity
      Heart muscle (at protein level).
    • Involvement in disease
      Cardiomyopathy, dilated 1P
      Cardiomyopathy, familial hypertrophic 18
    • Sequence similarities
      Belongs to the phospholamban family.
    • Post-translational
      modifications
      Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes.
    • Cellular localization
      Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. Mitochondrion membrane. Membrane. Colocalizes with HAX1 at the endoplasmic reticulum (PubMed:17241641). Colocalizes with DMPK a the sarcoplasmic reticulum (PubMed:15598648).
    • Information by UniProt
    • Database links
    • Alternative names
      • Cardiac phospholamban antibody
      • CMD1P antibody
      • CMH18 antibody
      • PLB antibody
      • Pln antibody
      • PPLA_HUMAN antibody
      see all

    Images

    • All lanes : Anti-Phospholamban (phospho S16) antibody [EPR1911] (ab92697) at 1/200000 dilution

      Lane 1 : Fetal heart lysate (untreated)
      Lane 2 : Fetal heart lysate (treated with LP)

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti-Rabbit antibody at 1/2000 dilution

      Predicted band size : 6 kDa

    References

    ab92697 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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