Anti-Phospholamban (phospho S16 + T17) antibody (ab62170)

Overview

  • Product nameAnti-Phospholamban (phospho S16 + T17) antibody
    See all Phospholamban primary antibodies
  • Description
    Rabbit polyclonal to Phospholamban (phospho S16 + T17)
  • SpecificityDetects endogenous levels of Phospholamban only when phosphorylated at serine 16 and threonine 17
  • Tested applicationsSuitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human Phospholamban around the phosphorylation site of serine 16 and threonine 17 (R-A-SP-TP-I-E).

  • Positive control
    • HuvEc cells

Properties

Applications

Our Abpromise guarantee covers the use of ab62170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 6 kDa. PubMed: 24386101
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.

Target

  • FunctionReversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation.
  • Tissue specificityHeart muscle (at protein level).
  • Involvement in diseaseCardiomyopathy, dilated 1P
    Cardiomyopathy, familial hypertrophic 18
  • Sequence similaritiesBelongs to the phospholamban family.
  • Post-translational
    modifications
    Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes.
  • Cellular localizationEndoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. Mitochondrion membrane. Membrane. Colocalizes with HAX1 at the endoplasmic reticulum (PubMed:17241641). Colocalizes with DMPK a the sarcoplasmic reticulum (PubMed:15598648).
  • Information by UniProt
  • Database links
  • Alternative names
    • Cardiac phospholamban antibody
    • CMD1P antibody
    • CMH18 antibody
    • PLB antibody
    • Pln antibody
    • PPLA_HUMAN antibody
    see all

Anti-Phospholamban (phospho S16 + T17) antibody images

  • Immunofluorescence analysis of HuvEc cells, using Phospholamban (phospho S16 + T17) antibody (ab62170) at 1/500 - 1/1000 dilution, in the presence (right panel) and absence (left panel) of immunising phosphopeptide.

References for Anti-Phospholamban (phospho S16 + T17) antibody (ab62170)

This product has been referenced in:
  • Huang Y  et al. Sarcoplasmic phospholamban protein is involved in the mechanisms of postresuscitation myocardial dysfunction and the cardioprotective effect of nitrite during resuscitation. PLoS One 8:e82552 (2013). WB ; Rat . Read more (PubMed: 24386101) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"