Recombinant Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102] (ab32354)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y102] to Phospholipase A2 activator protein (PLAP)
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102]
See all Phospholipase A2 activator protein (PLAP) primary antibodies -
Description
Rabbit monoclonal [Y102] to Phospholipase A2 activator protein (PLAP) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Phospholipase A2 activator protein (PLAP) aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- MCF-7 and K562 cell lysates and human lung metastatic cancer tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y102 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 80 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 80 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Involved in the maintenance of ubiquitin levels. -
Sequence similarities
Belongs to the WD repeat PLAP family.
Contains 6 ARM repeats.
Contains 1 PFU domain.
Contains 1 PUL domain.
Contains 7 WD repeats. -
Domain
The PUL domain is composed of 6 armadillo-like repeats and mediates the interaction with VCP C-terminus.
The PFU domain mediates interaction with ubiquitin. - Information by UniProt
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Database links
- Entrez Gene: 9373 Human
- Omim: 603873 Human
- SwissProt: Q9Y263 Human
- Unigene: 27182 Human
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Alternative names
- FLJ11281 antibody
- FLJ12699 antibody
- Glycerophosphatase antibody
see all
Images
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All lanes : Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102] (ab32354) at 1/1000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : K562 cell lysate
Predicted band size: 80 kDa
Observed band size: 81 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded human lung metastatic cancer using ab32354 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab32354 has been referenced in 4 publications.
- Hülsmann J et al. AP-SWATH Reveals Direct Involvement of VCP/p97 in Integrated Stress Response Signaling Through Facilitating CReP/PPP1R15B Degradation. Mol Cell Proteomics 17:1295-1307 (2018). PubMed: 29599191
- Lin Q et al. Inhibition of CD9 expression reduces the metastatic capacity of human hepatocellular carcinoma cell line MHCC97-H. Int J Oncol 53:266-274 (2018). PubMed: 29749468
- Zhang F et al. Alteration in the activation state of new inflammation-associated targets by phospholipase A2-activating protein (PLAA). Cell Signal 20:844-61 (2008). PubMed: 18291623
- Alexandru G et al. UBXD7 binds multiple ubiquitin ligases and implicates p97 in HIF1alpha turnover. Cell 134:804-16 (2008). PubMed: 18775313