Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102] (ab32354)


  • Product name
    Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102]
    See all Phospholipase A2 activator protein (PLAP) primary antibodies
  • Description
    Rabbit monoclonal [Y102] to Phospholipase A2 activator protein (PLAP)
  • Specificity
    ab32354 recognises Phospholipase A2 activator protein (PLAP).
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N-term of human PLAP.

  • Positive control
    • MCF-7 and K562 cell lysates and human lung metastatic cancer tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab32354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 80 kDa).
IHC-P 1/50 - 1/100.
ICC 1/50.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Involved in the maintenance of ubiquitin levels.
    • Sequence similarities
      Belongs to the WD repeat PLAP family.
      Contains 6 ARM repeats.
      Contains 1 PFU domain.
      Contains 1 PUL domain.
      Contains 7 WD repeats.
    • Domain
      The PUL domain is composed of 6 armadillo-like repeats and mediates the interaction with VCP C-terminus.
      The PFU domain mediates interaction with ubiquitin.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ11281 antibody
      • FLJ12699 antibody
      • Glycerophosphatase antibody
      • OTTHUMP00000045176 antibody
      • Phospholipase A 2 activating protein antibody
      • Phospholipase A-2-activating protein antibody
      • Phospholipase A2 activating protein antibody
      • PLA2P antibody
      • Plaa antibody
      • PLAP antibody
      • PLAP_HUMAN antibody
      see all

    Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102] images

    • All lanes : Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102] (ab32354) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : K562 cell lysate

      Predicted band size : 80 kDa
      Observed band size : 81 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded human lung metastatic cancer using ab32354 at 1/50 dilution.

    References for Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102] (ab32354)

    This product has been referenced in:
    • Zhang F  et al. Alteration in the activation state of new inflammation-associated targets by phospholipase A2-activating protein (PLAA). Cell Signal 20:844-61 (2008). Read more (PubMed: 18291623) »
    • Alexandru G  et al. UBXD7 binds multiple ubiquitin ligases and implicates p97 in HIF1alpha turnover. Cell 134:804-16 (2008). Read more (PubMed: 18775313) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your reply. I would be happy to provide a replacement vial of ab18184. To do so I will just need to access the original order on our system. Please provide the Abcam order reference number or an internal purchase order number so I can pro...

    Read More
    Western blot
    Human Cell lysate - whole cell (fibroblasts culture)
    Loading amount
    30 µg
    fibroblasts culture
    Gel Running Conditions
    Reduced Denaturing (10% SDS-page gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Dr. Yishay Shoval

    Verified customer

    Submitted Jan 04 2012

    Thank you for your enquiry. The exact location of the peptide is proprietary information but it is between aa 70 - 90. I hope this helps. Please do not hesitate to contact us if you have any more questions.


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