Anti-Cytosolic Phospholipase A2 antibody (ab104252)
Key features and details
- Goat polyclonal to Cytosolic Phospholipase A2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cytosolic Phospholipase A2 antibody
See all Cytosolic Phospholipase A2 primary antibodies -
Description
Goat polyclonal to Cytosolic Phospholipase A2 -
Host species
Goat -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Horse, Orangutan -
Immunogen
Synthetic peptide:
C-QEKTFRQQRKEHIR
, corresponding to internal sequence amino acids 153-166 of Human Phospholipase A2 (NP_077734.1). -
Positive control
- WB: HeLa cell lysates. ICC/IF: A549 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab104252 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab104252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 85 kDa.
1 hour primary incubation is recommended for this product. |
|
ICC/IF |
Use a concentration of 10 µg/ml.
|
|
IHC-P |
Use a concentration of 3.75 µg/ml.
|
Notes |
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WB
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 85 kDa. 1 hour primary incubation is recommended for this product. |
ICC/IF
Use a concentration of 10 µg/ml. |
IHC-P
Use a concentration of 3.75 µg/ml. |
Target
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Function
Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response. -
Tissue specificity
Expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 PLA2c domain. -
Domain
The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+). -
Post-translational
modificationsActivated by phosphorylation at both Ser-505 and Ser-727. -
Cellular localization
Cytoplasm. Cytoplasmic vesicle. Translocates to membrane vesicles in a calcium-dependent fashion. - Information by UniProt
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Database links
- Entrez Gene: 100033889 Horse
- Entrez Gene: 151056 Human
- Entrez Gene: 5321 Human
- Entrez Gene: 100173125 Orangutan
- Entrez Gene: 192259 Rat
- Omim: 600522 Human
- SwissProt: O77793 Horse
- SwissProt: P47712 Human
see all -
Alternative names
- Calcium dependent phospholipid binding protein antibody
- CPLA 2 antibody
- cPLA2 alpha antibody
see all
Images
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Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation with ab104252 for 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml) (green). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
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Anti-Cytosolic Phospholipase A2 antibody (ab104252) at 1 µg + HeLa cell lysate at 35 µg
Predicted band size: 85 kDa
Datasheets and documents
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Datasheet download
References (1)
ab104252 has been referenced in 1 publication.
- Tunset HM et al. Cytosolic Phospholipase A2 Alpha Regulates TLR Signaling and Migration in Metastatic 4T1 Cells. Int J Mol Sci 20:N/A (2019). PubMed: 31569627