Anti-Phospholipase A2 antibody (ab124230)

Overview

  • Product nameAnti-Phospholipase A2 antibody
    See all Phospholipase A2 primary antibodies
  • Description
    Goat polyclonal to Phospholipase A2
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse
  • Immunogen

    Synthetic peptide:

    C-QEKTFRQQRKEHIR

    conjugated to KLH, corresponding to internal sequence amino acids 153-166 of Human Phospholipase A2 (NP_077734.1).

  • Positive control
    • Human uterus tissue; HeLa cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab124230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 85 kDa).
ELISA 1/32000.
IHC-P Use a concentration of 3.75 µg/ml.

Target

  • FunctionSelectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.
  • Tissue specificityExpressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium.
  • Sequence similaritiesContains 1 C2 domain.
    Contains 1 PLA2c domain.
  • DomainThe N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+).
  • Post-translational
    modifications
    Activated by phosphorylation at both Ser-505 and Ser-727.
  • Cellular localizationCytoplasm. Cytoplasmic vesicle. Translocates to membrane vesicles in a calcium-dependent fashion.
  • Information by UniProt
  • Database links
  • Alternative names
    • Calcium dependent phospholipid binding protein antibody
    • CPLA 2 antibody
    • cPLA2 alpha antibody
    • cPLA2 antibody
    • Cytosolic phospholipase A2 antibody
    • Cytosolic phospholipase A2 group IVA antibody
    • Lysophospholipase antibody
    • MGC126350 antibody
    • PA24A_HUMAN antibody
    • Phosphatidylcholine 2 acylhydrolase antibody
    • Phosphatidylcholine 2-acylhydrolase antibody
    • Phospholipase A2 group 4 A antibody
    • Phospholipase A2 group IVA (cytosolic calcium dependent) antibody
    • Phospholipase A2 group IVA antibody
    • PhospholipaseA2 antibody
    • PLA2G4 antibody
    • pla2g4a antibody
    see all

Anti-Phospholipase A2 antibody images

  • Anti-Phospholipase A2 antibody (ab124230) at 3 µg/ml + HeLa cell lysate at 35 µg

    Predicted band size : 85 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour
  • ab124230, at 3.75 µg/ml, staining Phospholipase A2 in formalin fixed, paraffin embedded Human uterus tissue by Immunohistochemistry.

References for Anti-Phospholipase A2 antibody (ab124230)

ab124230 has not yet been referenced specifically in any publications.

Product Wall

Vielen Dank für Ihren Anruf.

Das Labor, das Anti-Phospholipase A2Antikoerper ab23705 herstellt, hat bestätigt, dass das Immunogen das Volllängenprotein sPLA2 (human Type IIA) mit der SwissProtID P14555.2 ist. Damit ist dieser A...

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The anti-Phospholipase A2 antibody (ab124230) referenced in the email you received has as yet only been tested in Western blotting, ELISA and immunohistochemistry of paraffi...

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