Overview

  • Product name
    Anti-Phospholipase A2 antibody
  • Description
    Rabbit polyclonal to Phospholipase A2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Rat, Human
    Does not react with: Rabbit, Cow, Pig
  • Immunogen

    Human recombinant sPLA2 (Type IIa).

Properties

Applications

Our Abpromise guarantee covers the use of ab23705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 14 kDa.
IP Use at an assay dependent concentration.

Target

  • Function
    Thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
  • Sequence similarities
    Belongs to the phospholipase A2 family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • GIIC sPLA2 antibody
      • Group IIA phospholipase A2 antibody
      • membrane associated antibody
      • MOM1 antibody
      • Non-pancreatic secretory phospholipase A2 antibody
      • NPS-PLA2 antibody
      • OTTHUMP00000002786 antibody
      • PA2GA_HUMAN antibody
      • Phosphatidylcholine 2-acylhydrolase 2A antibody
      • Phospholipase A2 antibody
      • Phospholipase A2 group IIA antibody
      • Phospholipase A2, group IIA (platelets, synovial fluid) antibody
      • PLA2 antibody
      • PLA2B antibody
      • PLA2G2A antibody
      • PLA2L antibody
      • PLA2S antibody
      • PLAS1 antibody
      • RASF A antibody
      • sPLA2 antibody
      see all

    Images

    • Western blot - Anti-Phospholipase A2 antibody (ab23705)
      Western blot - Anti-Phospholipase A2 antibody (ab23705)

      Western blot detection of human secretory phospholipase A2 (25µg)

      ab23705 at 1/1000 western blot detection of 0.25 ug of human secretory phospolipase A2.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase A2 antibody (ab23705)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase A2 antibody (ab23705)
      IHC image of ab23705 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23705, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References

    This product has been referenced in:
    • Wang J  et al. Qishenyiqi Dropping Pill attenuates myocardial fibrosis in rats by inhibiting RAAS-mediated arachidonic acid inflammation. J Ethnopharmacol 176:375-84 (2015). WB ; Rat . Read more (PubMed: 26590099) »
    • Pernet E  et al. Pseudomonas aeruginosa eradicates Staphylococcus aureus by manipulating the host immunity. Nat Commun 5:5105 (2014). IHC ; Human . Read more (PubMed: 25290234) »

    See all 5 Publications for this product

    Publishing research using ab23705? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    7 reviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Phospholipase A2 antibody

     Good
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Monkey Tissue sections (Gingiva)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
    Permeabilization
    No
    Specification
    Gingiva
    Blocking step
    Generic block reagent as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Jan 25 2017

    ich suche einen AK, der alle Isoformen der Phospholipase 2A erkennt (humane Haut, IHC-P, evtl -Fr, Wb).

    Vielen Dank für Ihren Anruf.

    Das Labor, das Anti-Phospholipase A2Antikoerper ab23705 herstellt, hat bestätigt, dass das Immunogen das Volllängenprotein sPLA2 (human Type IIA) mit der SwissProtID P14555.2 ist. Damit ist dieser A...

    Read More

    Do you have an in-house support for the use of this antibody in various formalin-fixed paraffin-embedded tissues? I am particularly interested in the brain. Thanks. I have searched the literature and found several articles for your antibody success in ...

    Read More

    Thank you for contacting Abcam.

    Under our Abpromise we guarantee our products to work as stated on the datasheet and if they do not work as stated, and we cannot resolve the issue you are having with the antibody then we would be happy to eit...

    Read More

    Hi. I have another question and no one local to tap their expertise in IHC. In your technical bulletin for IHC protocols you are using Tris/Triton X-100 for permeabilization/washes. I have been using a Tween20/Tris buffer for my washes. One article...

    Read More

    Many of the protocols out there will call for "either Triton-X or Tween20" for permeabilization/washes and I think either would be a good choice. The advantage to Tween20 is that it is more gentle and less likely to disrupt the cell, however Triton-X i...

    Read More

    Hi. I am inquiring about the sPLA2 rabbit poly-clonal antibody and its use in IHC-P. I have attempted numerous studies on human brain tissues using sPLA2 antibodies from other vendors and have not had consistent results and am considering trying...

    Read More

    Thank you for contacting us. This product should work well with your conditions but of course must be optimized by the customer. This product was tested with short incubation time but may be used overnight at 4 degrees if you find you need a stronger s...

    Read More

    No specific band in Western blot.

    Thank you for your phone call today and letting us know about the problem with ab23705. Your credit note ID is ***. I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the origina...

    Read More

    I am interested by your antibody directed against secretory PLA2. I want to investigate the level of expression of sPLA2 type II by Western Blot in rat tissue. Would this antibody be suitable ?

    Thank you for your enquiry. Yes, this antibody does appear to be suitable for your needs. Ab23705 has been tested in Western blotting with rat samples and the antibody recognizes type IIa. If you have any additional questions, please contact us again.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up