Overview

  • Product name
    Anti-Phospholipase A2 X antibody
    See all Phospholipase A2 X primary antibodies
  • Description
    Rabbit polyclonal to Phospholipase A2 X
  • Host species
    Rabbit
  • Specificity
    This antibody is specific for Phospholipase A2 X.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human Phospholipase A2 X. A 15.5 kDa protein containing 123 amino acid residues of the human Phospholipase A2 X and 16 additional amino acid residues, HisTag. Source of immunogen, E.coli

Properties

Applications

Our Abpromise guarantee covers the use of ab47111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.

Target

  • Function
    PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Has a powerful potency for releasing arachidonic acid from cell membrane phospholipids. Prefers phosphatidylethanolamine and phosphatidylcholine liposomes to those of phosphatidylserine.
  • Tissue specificity
    Found in spleen, thymus, peripheral blood leukocytes, pancreas, lung, and colon.
  • Sequence similarities
    Belongs to the phospholipase A2 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • Group 10 secretory phospholipase A2 antibody
      • Group X secretory phospholipase A2 antibody
      • GX sPLA2 antibody
      • GXPLA2 antibody
      • GXSPLA2 antibody
      • PA2GX_HUMAN antibody
      • Phosphatidylcholine 2 acylhydrolase GX antibody
      • Phosphatidylcholine 2-acylhydrolase 10 antibody
      • Phospholipase A2 group X antibody
      • PLA2G10 antibody
      • SPLA2 antibody
      • sPLA2-X antibody
      • sPLA2X antibody
      see all

    Images

    • Western blot - Anti-Phospholipase A2 X antibody (ab47111)
      Western blot - Anti-Phospholipase A2 X antibody (ab47111)Image from Curfs DM et al, J Biol Chem. 2008 Aug 1;283(31):21640-8. Epub 2008 May 28, Fig 1.DOI 10.1074/jbc.M710584200
      All lanes : Anti-Phospholipase A2 X antibody (ab47111) at 1 µg/ml

      Lane 1 : Whole cell extracts from RAW264.7 control cells
      Lane 2 : Whole cell extracts from RAW264.7 cells overexpressing Phospholipase A2 X

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Horseradish peroxidase-conjugated secondary antibody

      Predicted band size: 17 kDa
      Observed band size: 17 kDa

    References

    This product has been referenced in:
    • Curfs DM  et al. Macrophage Secretory Phospholipase A2 Group X Enhances Anti-inflammatory Responses, Promotes Lipid Accumulation, and Contributes to Aberrant Lung Pathology. J Biol Chem 283:21640-8 (2008). WB ; Human . Read more (PubMed: 18511424) »

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