Recombinant
RabMAb

Anti-Phospholipase A2 X antibody [EPR11202] (ab166634)

Overview

  • Product name
    Anti-Phospholipase A2 X antibody [EPR11202]
    See all Phospholipase A2 X primary antibodies
  • Description
    Rabbit monoclonal [EPR11202] to Phospholipase A2 X
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A recombinant protein fragment corresponding to a region of Human Phospholipase A2 X (UniProt ID: O15496).

  • Positive control
    • TT, A549, MCF7 and Caco 2 cell lysates; Human pancreas and tonsil tissues; permeabilized A549 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab166634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 17 kDa.
IHC-P 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function
      PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Has a powerful potency for releasing arachidonic acid from cell membrane phospholipids. Prefers phosphatidylethanolamine and phosphatidylcholine liposomes to those of phosphatidylserine.
    • Tissue specificity
      Found in spleen, thymus, peripheral blood leukocytes, pancreas, lung, and colon.
    • Sequence similarities
      Belongs to the phospholipase A2 family.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Group 10 secretory phospholipase A2 antibody
      • Group X secretory phospholipase A2 antibody
      • GX sPLA2 antibody
      • GXPLA2 antibody
      • GXSPLA2 antibody
      • PA2GX_HUMAN antibody
      • Phosphatidylcholine 2 acylhydrolase GX antibody
      • Phosphatidylcholine 2-acylhydrolase 10 antibody
      • Phospholipase A2 group X antibody
      • PLA2G10 antibody
      • SPLA2 antibody
      • sPLA2-X antibody
      • sPLA2X antibody
      see all

    Images

    • All lanes : Anti-Phospholipase A2 X antibody [EPR11202] (ab166634) at 1/1000 dilution

      Lane 1 : TT cell lysate
      Lane 2 : A549 cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : Caco 2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 17 kDa

    • Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling Phospholipase A2 X with ab166634 at a 1/100 dilution.
    • Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Phospholipase A2 X with ab166634 at a 1/100 dilution.
    • Flow cytometric analysis of permeabilized A549 cells using ab166634 at a 1/100 dilution (red) or a rabbit IgG (negative) (green).

    References

    This product has been referenced in:
    • Nolin JD  et al. Secreted PLA2 group X orchestrates innate and adaptive immune responses to inhaled allergen. JCI Insight 2:N/A (2017). WB ; Mouse . Read more (PubMed: 29093264) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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