• Product nameAnti-Phospholipase C beta 3 antibody
    See all Phospholipase C beta 3 primary antibodies
  • Description
    Rabbit polyclonal to Phospholipase C beta 3
  • SpecificityThis antibody detects endogenous levels of total Phospholipase C beta 3 protein.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthetic non-phosphopeptide designed around the phosphorylation site of serine 1105, of human Phospholipase C beta 3.

  • Positive control
    • Human brain tissue (IHC).



Our Abpromise guarantee covers the use of ab52199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 137 kDa (predicted molecular weight: 137 kDa).
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.


  • FunctionThe production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
  • Sequence similaritiesContains 1 C2 domain.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 antibody
    • 1-phosphatidylinositol-4 antibody
    • 5-bisphosphate phosphodiesterase beta-3 antibody
    • Phosphoinositide phospholipase C antibody
    • Phosphoinositide phospholipase C-beta-3 antibody
    • Phospholipase C beta 3 antibody
    • Phospholipase C beta 3 (phosphatidylinositol specific) antibody
    • Phospholipase C-beta-3 antibody
    • PLC beta 3 antibody
    • PLC-beta-3 antibody
    • Plcb3 antibody
    • PLCB3_HUMAN antibody
    see all

Anti-Phospholipase C beta 3 antibody images

  • Paraffin-embedded human brain tissue stained for phospholipase C beta 3, using ab52199 (1/100). The right hand panel represents a negative control in which ab52199 has been pre-incubated with the immunizing (blocking) peptide.
  • All lanes : Anti-Phospholipase C beta 3 antibody (ab52199) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells treated with PMA (125ng/ml, 30min).
    Lane 2 : Extracts from HeLa cells treated with PMA (125ng/ml, 30min). For this panel, ab52199 was pre-incubated with the immunizing (blocking) peptide.

    Predicted band size : 137 kDa
    Observed band size : 137 kDa

References for Anti-Phospholipase C beta 3 antibody (ab52199)

This product has been referenced in:
  • Béziau DM  et al. Expression of phosphoinositide-specific phospholipase C isoforms in native endothelial cells. PLoS One 10:e0123769 (2015). WB, IHC-P . Read more (PubMed: 25875657) »
  • Feng J  et al. Thromboxane-induced contractile response of human coronary arterioles is diminished after cardioplegic arrest. Ann Thorac Surg 92:829-36 (2011). WB ; Human . Read more (PubMed: 21871266) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (5-16% gel)
Sample Human Cell lysate - whole cell (Hela cells)
Specification Hela cells
Treatment see additional notes
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 01 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer (pH6.0)
Sample Human Tissue sections (lung)
Specification lung
Permeabilization No
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 31 2014