Recombinant
RabMAb

Anti-Phospholipase C beta 3 antibody [EPR5951] (ab124735)

Overview

  • Product name
    Anti-Phospholipase C beta 3 antibody [EPR5951]
    See all Phospholipase C beta 3 primary antibodies
  • Description
    Rabbit monoclonal [EPR5951] to Phospholipase C beta 3
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human Phospholipase C beta 3.

  • Positive control
    • HCT 116, 293T, and A431 cell lysates; Human breast and colon tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab124735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
    • Sequence similarities
      Contains 1 C2 domain.
      Contains 1 PI-PLC X-box domain.
      Contains 1 PI-PLC Y-box domain.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 antibody
      • 1-phosphatidylinositol-4 antibody
      • 5-bisphosphate phosphodiesterase beta-3 antibody
      • Phosphoinositide phospholipase C antibody
      • Phosphoinositide phospholipase C-beta-3 antibody
      • Phospholipase C beta 3 antibody
      • Phospholipase C beta 3 (phosphatidylinositol specific) antibody
      • Phospholipase C-beta-3 antibody
      • PLC beta 3 antibody
      • PLC-beta-3 antibody
      • Plcb3 antibody
      • PLCB3_HUMAN antibody
      see all

    Images

    • All lanes : Anti-Phospholipase C beta 3 antibody [EPR5951] (ab124735) at 1/1000 dilution

      Lane 1 : HCT 116 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 139 kDa
    • ab124735 at 1/100 dilution staining Phospholipase C beta 3 in paraffin-embedded Human breast tissue by Immunohistochemistry.
    • ab124735 at 1/100 dilution staining Phospholipase C beta 3 in paraffin-embedded Human colon tissue by Immunohistochemistry.

    References

    ab124735 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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