Anti-Phospholipase C beta 3 antibody [EPR5951] (ab124735)


  • Product nameAnti-Phospholipase C beta 3 antibody [EPR5951]
    See all Phospholipase C beta 3 primary antibodies
  • Description
    Rabbit monoclonal [EPR5951] to Phospholipase C beta 3
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human Phospholipase C beta 3.

  • Positive control
    • HCT 116, 293T, and A431 cell lysates; Human breast and colon tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab124735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • FunctionThe production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
    • Sequence similaritiesContains 1 C2 domain.
      Contains 1 PI-PLC X-box domain.
      Contains 1 PI-PLC Y-box domain.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 antibody
      • 1-phosphatidylinositol-4 antibody
      • 5-bisphosphate phosphodiesterase beta-3 antibody
      • Phosphoinositide phospholipase C antibody
      • Phosphoinositide phospholipase C-beta-3 antibody
      • Phospholipase C beta 3 antibody
      • Phospholipase C beta 3 (phosphatidylinositol specific) antibody
      • Phospholipase C-beta-3 antibody
      • PLC beta 3 antibody
      • PLC-beta-3 antibody
      • Plcb3 antibody
      • PLCB3_HUMAN antibody
      see all

    Anti-Phospholipase C beta 3 antibody [EPR5951] images

    • All lanes : Anti-Phospholipase C beta 3 antibody [EPR5951] (ab124735) at 1/1000 dilution

      Lane 1 : HCT 116 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 139 kDa
    • ab124735 at 1/100 dilution staining Phospholipase C beta 3 in paraffin-embedded Human breast tissue by Immunohistochemistry.
    • ab124735 at 1/100 dilution staining Phospholipase C beta 3 in paraffin-embedded Human colon tissue by Immunohistochemistry.

    References for Anti-Phospholipase C beta 3 antibody [EPR5951] (ab124735)

    ab124735 has not yet been referenced specifically in any publications.

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